1887

Abstract

A polyphasic taxonomic study was conducted on strain HK31, a moderately halophilic bacterium isolated from a solar saltern in Chefchaouen, Morocco. The strain was a Gram-reaction-negative, oxidase-positive rod, which was motile by means of peritrichous flagella. The strain required NaCl for growth and grew in salt concentrations (mixture of sea salts) of 0.5–20 % (w/v) (optimum 5–7.5 %, w/v), at 25–45 °C (optimum 32 °C) and at pH 5–10 (optimum pH 6–9). Strain HK31 did not produce acids from sugars and its metabolism was respiratory, using oxygen as terminal electron acceptor. The strain was positive for the accumulation of poly-β-hydroxyalkanoate granules and formed mucoid colonies due to the excretion of an exopolysaccharide. The DNA G+C content was 61.5 mol%. 16S rRNA gene sequence analysis indicated that it belonged to the genus in the class . The most phylogenetically related species was , with which strain HK31 showed a 16S rRNA gene sequence similarity of 96.48 %. Its major fatty acids were Cω7, C, C cyclo ω8 , Cω7/iso-C 2-OH and C 3-OH and the predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q-9). Based on the evidence provided in this study, strain HK31 ( = CECT 7698  = LMG 25695) represents a novel species of the genus , for which the name is proposed.

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2011-11-01
2020-01-21
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Transmission electron micrograph of a cell of strain HK31T stained with ruthenium red.

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