1887

Abstract

An aerobic, saccharolytic, obligately thermophilic, motile, non-spore-forming bacterium, strain T49, was isolated from geothermally heated soil at Hell’s Gate, Tikitere, New Zealand. On the basis of 16S rRNA gene sequence similarity, T49 is the first representative of a new class in the newly described phylum , formerly known as candidate division OP10. Cells of strain T49 stained Gram-negative and were catalase-positive and oxidase-negative. Cells possessed a highly corrugated outer membrane. The major fatty acids were 16 : 0, i17 : 0 and ai17 : 0. The G+C content of the genomic DNA was 54.6 mol%. Strain T49 grew at 50–73 °C with an optimum temperature of 68 °C, and at pH 4.7–5.8 with an optimum growth pH of 5.3. A growth rate of 0.012 h was observed under optimal temperature and pH conditions. The primary respiratory quinone was MK-8. Optimal growth was achieved in the absence of NaCl, although growth was observed at NaCl concentrations as high as 2 % (w/v). Strain T49 was able to utilize mono- and disaccharides such as cellobiose, lactose, mannose and glucose, as well as branched or amorphous polysaccharides such as starch, CM-cellulose, xylan and glycogen, but not highly linear polysaccharides such as crystalline cellulose or cotton. On the basis of its phylogenetic position and phenotypic characteristics, we propose that strain T49 represents a novel bacterial genus and species within the new class classis nov. of the phylum . The type strain of gen. nov., sp. nov. is T49 ( = DSM 23976 = ICMP 18418).

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2011-10-01
2019-10-20
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vol. , part 10, pp. 2482–2490

Phenotypic comparison of strains YO-36 and T49

Strain T49 colonies on gellen-based solid medium, and pink/rose-coloured pellicle formed via planktonically grown T49 cells left to settle

Maximum-likelihood dendrogram based on 16S rRNA gene sequences of strain T49 and environmental clones belonging to and candidate division WS1

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