1887

Abstract

A group of 42 isolates were isolated from the soil of several disused hay fields, in the Drentse A agricultural research area (The Netherlands), that were taken out of production at different times. The group represents hitherto-uncultured lineages that have previously been found, by a non-cultural method, to be predominant in soil. The strains were subjected to a polyphasic taxonomic study, including (GTG)-PCR, 16S rDNA sequence analysis, DNA–DNA hybridizations, DNA base-ratio determination, fatty acid analysis and morphological and biochemical characterization. By comparing the groupings obtained by (GTG)-PCR and 16S rDNA sequence analysis, six clusters of similar strains could be recognized. A DNA–DNA relatedness study showed that these clusters represented five novel genospecies. Further analysis supported the proposal of five novel species in the genus , namely sp. nov. (type strain IDA3307=R-15439=LMG 21837=DSM 15603), sp. nov. (type strain IDA3632=R-15447=LMG 21834=DSM 15602), sp. nov. (type strain IDA0086=R-16300=LMG 21838=DSM 15604), sp. nov. (type strain IDA1115=R-16315=LMG 21833=DSM 15601) and sp. nov. (type strain IDA1967=R-16337=LMG 21831=DSM 15600).

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2004-01-01
2019-10-23
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