1887

Abstract

Five novel Gram-reaction-negative aerobic marine bacterial strains with DNA G+C contents <50 mol% were isolated from the seagrass . 16S rRNA sequence analysis indicated that they belonged to the genus . Major fatty acid compositions, comprising C 3-OH, C, Cω7 and Cω7, supported the affiliation of these strains to the genus . Strains IVIA-Po-14b, IVIA-Po-145 and IVIA-Po-155 were closely related to 46-16, according to phylogenetic analysis. However, DNA–DNA hybridization values <35 % among these strains revealed that they represented different species. Further differences in the phenotypes and minor fatty acid compositions were also found among the strains. Another two strains, designated IVIA-Po-181 and IVIA-Po-159, were found to be closely related to DSW10-10 but DNA–DNA relatedness levels <40 % in pairwise comparisons, as well as some additional differences in phenotypes and fatty acid compositions supported the creation of two novel species. Accordingly, strains IVIA-Po-14b( = CECT 7730  = NCIMB 14671), IVIA-Po-145 ( = CECT 7377  = NCIMB 14431), IVIA-Po-155 ( = CECT 7731  = NCIMB 14672), IVIA-Po-181 ( = CECT 7376  = NCIMB 14433) and IVIA-Po-159 ( = CECT 7732  = NCIMB 14673) represent novel species, for which the names sp. nov., sp. nov., sp. nov., sp. nov. and sp. nov. are proposed, respectively.

Funding
This study was supported by the:
  • Seneca Foundation, Autonomous Government of the Region of Murcia (Award 11867/PI/09)
  • Fundación BBVA
  • ESF
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.027227-0
2011-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/9/2191.html?itemId=/content/journal/ijsem/10.1099/ijs.0.027227-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  2. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–429[PubMed]
    [Google Scholar]
  3. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  4. Chang H. W., Roh S. W., Kim K. H., Nam Y. D., Yoon J. H., Oh H. M., Bae J. W. 2008; Marinomonas basaltis sp. nov., a marine bacterium isolated from black sand. Int J Syst Evol Microbiol 58:2743–2747 [View Article][PubMed]
    [Google Scholar]
  5. Chimetto L. A., Cleenwerck I., Brocchi M., Willems A., De Vos P., Thompson F. L. 2011; Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis. . Int J Syst Evol Microbiol 61:1170–1175 [CrossRef]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  7. Espinosa E., Marco-Noales E., Gómez D., Lucas-Elío P., Ordax M., Garcías-Bonet N., Duarte C. M., Sanchez-Amat A. 2010; Taxonomic study of Marinomonas strains isolated from the seagrass Posidonia oceanica, with descriptions of Marinomonas balearica sp. nov. and Marinomonas pollencensis sp. nov.. Int J Syst Evol Microbiol 60:93–98 [View Article][PubMed]
    [Google Scholar]
  8. Gupta P., Chaturvedi P., Pradhan S., Delille D., Shivaji S. 2006; Marinomonas polaris sp. nov., a psychrohalotolerant strain isolated from coastal sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 56:361–364 [View Article][PubMed]
    [Google Scholar]
  9. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  10. Ivanova E. P., Onyshchenko O. M., Christen R., Lysenko A. M., Zhukova N. V., Shevchenko L. S., Kiprianova E. A. 2005; Marinomonas pontica sp. nov., isolated from the Black Sea. Int J Syst Evol Microbiol 55:275–279 [View Article][PubMed]
    [Google Scholar]
  11. Johnson J. L. 1994; Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology pp. 655–682 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Kumura K., Minamishima Y., Yamamoto S., Ohashi N., Tamura A. 1991; DNA base composition of Rickettsia tsutsugamushi determined by reversed-phase high-performance liquid chromatography. Int J Syst Bacteriol 41:247–248 [View Article][PubMed]
    [Google Scholar]
  13. Lau K. W. K., Ren J. P., Wai N. L. M., Lau S. C. L., Qian P. Y., Wong P. K., Wu M. 2006; Marinomonas ostreistagni sp. nov., isolated from a pearl-oyster culture pond in Sanya, Hainan Province, China. Int J Syst Evol Microbiol 56:2271–2275 [View Article][PubMed]
    [Google Scholar]
  14. Macián M. C., Arahal D. R., Garay E., Pujalte M. J. 2005; Marinomonas aquamarina sp. nov., isolated from oysters and seawater. Syst Appl Microbiol 28:145–150 [View Article][PubMed]
    [Google Scholar]
  15. Martínez-Murcia A. J., Antón A. I., Rodríguez-Valera F. 1999; Patterns of sequence variation in two regions of the 16S rRNA multigene family of Escherichia coli . Int J Syst Bacteriol 49:601–610 [View Article][PubMed]
    [Google Scholar]
  16. Prabagaran S. R., Suresh K., Manorama R., Delille D., Shivaji S. 2005; Marinomonas ushuaiensis sp. nov., isolated from coastal sea water in Ushuaia, Argentina, sub-Antarctica. Int J Syst Evol Microbiol 55:309–313 [View Article][PubMed]
    [Google Scholar]
  17. Romanenko L. A., Uchino M., Mikhailov V. V., Zhukova N. V., Uchimura T. 2003; Marinomonas primoryensis sp. nov., a novel psychrophile isolated from coastal sea-ice in the Sea of Japan. Int J Syst Evol Microbiol 53:829–832 [View Article][PubMed]
    [Google Scholar]
  18. Romanenko L. A., Tanaka N., Frolova G. M. 2009; Marinomonas arenicola sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 59:2834–2838 [View Article][PubMed]
    [Google Scholar]
  19. Sanchez-Amat A., Solano F. 2005; Genus Marinomonas Van Landschoot and De Ley 1984, 91 VP (Effective publication Van Landschoot and De Ley 1983, 3071). In Bergey’s Manual of Systematic Bacteriology vol. 2 pp. 284–289 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  20. Solano F., Sanchez-Amat A. 1999; Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov.. Int J Syst Bacteriol 49:1241–1246 [View Article][PubMed]
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  22. Van Landschoot A., De Ley J. 1983; Intra- and intergenic similarities of the rRNA cistrons of Alteromonas, Marinomonas (gen. nov.) and some other Gram-negative bacteria. J Gen Microbiol 129:3057–3074
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  24. Yoon J. H., Kang S. J., Oh T. K. 2005; Marinomonas dokdonensis sp. nov., isolated from sea water. Int J Syst Evol Microbiol 55:2303–2307 [View Article][PubMed]
    [Google Scholar]
  25. Zhang D. C., Li H. R., Xin Y. H., Liu H. C., Chen B., Chi Z. M., Zhou P. J., Yu Y. 2008; Marinomonas arctica sp. nov., a psychrotolerant bacterium isolated from the Arctic. Int J Syst Evol Microbiol 58:1715–1718 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.027227-0
Loading
/content/journal/ijsem/10.1099/ijs.0.027227-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error