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Abstract

A novel species was identified based on analysis of 16S rRNA and (macrophage infectivity potentiator) gene sequences, cellular fatty acids, isoprenoid quinones, biochemical reactions, antigens and quantitative DNA–DNA hybridization. Strain CDC-1796-JAP-E was isolated from well water at the Nagasaki Municipal Medical Center, Japan. Two strains, CDC-3041-AUS-E and CDC-3558-AUS-E, were isolated from water samples during an outbreak of legionellosis in South Australia. Strain CDC-5427-OH-H was isolated from a 66-year-old female patient diagnosed with Legionnaires’ disease in the US. Cells from these four strains were Gram-negative, non-fluorescent, rod-shaped, and positive for alkaline phosphatase, esterase, leucine arylamidase, catalase, gelatinase, β-lactamase and tyrosine browning assay. Phylogenetic analysis of 16S rRNA and genes revealed that the four strains formed a distinct cluster within the genus The bacteria contained branched-chain fatty acids and quinones that are typical of members of the genus . Slide agglutination tests demonstrated no cross-reaction with 52 previously described members of the . DNA–DNA hybridization studies indicated that DNAs from the four strains were highly related (78–84 %) but they showed 29 % relatedness to ATCC 33761 and less than 10 % to strains of other species tested. These characterizations suggest that the isolates represent a novel species, for which the name sp. nov. is proposed; the type strain is CDC-1796-JAP-E ( = ATCC BAA-1557 = JCM 15315).

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2012-02-01
2021-03-05
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