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Abstract

A Gram-staining-positive, filamentous bacterial strain, designated A-T 0308, was isolated from soil of a tropical mangrove forest in Thailand. Strain A-T 0308 developed spherical sporangia containing non-motile spores on aerial mycelium. The novel strain contained -diaminopimelic acid, -acetyl-type peptidoglycan and madurose, mannose, ribose, galactose and glucose as whole-cell sugars. The predominant menaquinones were MK-9(H) and MK-9(H); a small amount of MK-9(H) and MK-9 was also detected. Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and phosphoglycolipid. The predominant cellular fatty acids were iso-C and 10-methylated C. The GC content of the DNA was 72 mol%. Phenotypic and chemotaxonomic analyses showed that the novel isolate had characteristics typical of members of the genus . 16S rRNA gene sequence analysis also indicated that the strain belongs to the genus and that it represents a clade distinct from other members of the genus with sequence similarities ranging from 96.3 to 97.8 % between the novel strain and its closest relatives. Based on the results of phenotypic, chemotaxonomic and phylogenetic studies, strain A-T 0308 ( = BCC 21702  = NBRC 107571) represents a novel species of the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • Novartis Pharma AG
  • Bioresources Research Network (BRN), Bangkok, Thailand
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2011-12-01
2021-08-03
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