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Abstract

A Gram-staining-positive, filamentous bacterial strain, designated A-T 0308, was isolated from soil of a tropical mangrove forest in Thailand. Strain A-T 0308 developed spherical sporangia containing non-motile spores on aerial mycelium. The novel strain contained -diaminopimelic acid, -acetyl-type peptidoglycan and madurose, mannose, ribose, galactose and glucose as whole-cell sugars. The predominant menaquinones were MK-9(H) and MK-9(H); a small amount of MK-9(H) and MK-9 was also detected. Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and phosphoglycolipid. The predominant cellular fatty acids were iso-C and 10-methylated C. The GC content of the DNA was 72 mol%. Phenotypic and chemotaxonomic analyses showed that the novel isolate had characteristics typical of members of the genus . 16S rRNA gene sequence analysis also indicated that the strain belongs to the genus and that it represents a clade distinct from other members of the genus with sequence similarities ranging from 96.3 to 97.8 % between the novel strain and its closest relatives. Based on the results of phenotypic, chemotaxonomic and phylogenetic studies, strain A-T 0308 ( = BCC 21702  = NBRC 107571) represents a novel species of the genus , for which the name sp. nov. is proposed.

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2011-12-01
2019-10-22
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vol. , part 12, pp. 2890 - 2894

Polar lipid profiles of strain A-T0308 separated by 2-dimensional TLC and detection with molybdatophosphoric acid.

Phylogenetic trees derived from 16S rRNA gene sequences reconstructed using neighbour-joining, maximum-parsimony and maximum-likelihood methods showing the relationships between A-T 0308 and members of the family .

Cellular fatty acid compositions of A-T 0308 and the six recognized species of the genus .

DNA-DNA relatedness (mean of three replications) among the isolates A-T 0308 with the type strains 3-28(8) , 3D-72(35) , DSM 44094 , DSM 43801 , YIM 48771 and YIM 48782 .

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