sp. nov., isolated from effective nodules of and Free

Abstract

Five strains isolated from root nodules of and grown in the Anhui and Sichuan provinces of China were classified as members of the genus . These strains had identical 16S rRNA gene sequences which shared 99.48 %, 99.48 % and 99.22 % similarity with the most closely related strains of PAC68, PAC48 and USDA 76, respectively. A study using a polyphasic approach, including 16S rRNA gene RFLP, IGS-RFLP, BOX-PCR, comparative sequence analysis of the 16S–23S rRNA intergenic spacer (IGS) and the , and genes, DNA–DNA hybridization and phenotypic tests, showed that the five strains clustered into a coherent group that differentiated them from all recognized species of the genus . Sequencing of and genes and cross-nodulation tests showed that the representative strains CCBAU 23086, CCBAU 23160 and CCBAU 61434, isolated from different plants, had identical and gene sequences and were all able to nodulate , and . Based upon these results, the name sp. nov. is proposed for this novel species and strain CCBAU 23086 ( = LMG 25572 = HAMBI 3052) is designated as the type strain. The DNA GC mol% is 60.14 ( ).

Funding
This study was supported by the:
  • Foundation of the State Key Basic Research and Development Plan of China (Award 2010CB126500)
  • National Natural Science Foundation of China (Award 30970004)
  • National Project for Basic S&T Platform Construction (Award 2005DKA21201-10)
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2011-10-01
2024-03-29
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