1887

Abstract

A format for the description of single novel species is proposed, which should facilitate the reviewing process by assisting the provision of data in a standardized form. , , , , A Gram-negative, rod-shaped, non-spore-forming bacterium (Iso 196) was isolated from chicken faeces. On the basis of 16S rRNA gene sequence similarity, strain Iso 196 was shown to belong to the -2 subclass of the related to (95·6 %), (95·0 %) and (94·6 %), and the phylogenetic distance from any validly described species within the genus was less than 95 %. Chemotaxonomic data (major ubiquinone – Q-10; major polyamines – spermidine and putrescine; major polar lipids – phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine; major fatty acids – C 7 and C cyclo 8) supported the affiliation of strain Iso 196 to the genus . The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Iso 196 from the four validly published species. Iso 196 therefore represents a new species, for which the name sp. nov. is proposed, with the type strain Iso 196 (=DSM 15295 =CIP 107753).

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2003-05-01
2019-08-18
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References

  1. Altenburger, P., Busse, H.-J., Kämpfer, P., Lubitz, W. & Makristathis, A. ( 1996; ). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef]
    [Google Scholar]
  2. Busse, H.-J. & Auling, G. ( 1988; ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef]
    [Google Scholar]
  3. Busse, H.-J., Bunka, S., Hensel, A. & Lubitz, W. ( 1997; ). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47, 698–708.[CrossRef]
    [Google Scholar]
  4. Choma, A. & Komaniecka, I. ( 2002; ). Analysis of phospholipids and ornithine-containing lipids from Mesorhizobium spp. Syst Appl Microbiol 25, 326–331.[CrossRef]
    [Google Scholar]
  5. Geiger, O., Röhrs, V., Weissenmayer, B., Finan, T. M. & Thomas-Oates, J. E. ( 1999; ). The regulator gene phoB mediates phosphate stress-controlled synthesis of the membrane lipid diacylglyceryl-N,N,N-trimethylhomoserine in Rhizobium (Sinorhizobium) meliloti. Mol Microbiol 32, 63–73.[CrossRef]
    [Google Scholar]
  6. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  7. Hamana, K. & Takeuchi, M. ( 1998; ). Polyamine profiles as chemotaxonomic markers within alpha, beta, gamma, delta, and epsilon subclasses of class Proteobacteria: distribution of 2-hydroxyputrescine and homospermidine. Microbiol Cult Coll 14, 1–14.
    [Google Scholar]
  8. Holmes, B., Popoff, M., Kiredjian, M. & Kersters, K. ( 1988; ). Ochrobactrum anthropi gen. nov., sp. nov. from human clinical specimens and previously known as Group Vd. Int J Syst Bacteriol 38, 406–416.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Steiof, M. & Dott, W. ( 1991; ). Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef]
    [Google Scholar]
  11. Kämpfer, P., Müller, C., Mau, M., Neef, A., Auling, G., Busse, H.-J., Osborn, A. M. & Stolz, A. ( 1999; ). Description of Pseudaminobacter gen. nov. with two new species, Pseudaminobacter salicylatoxidans sp. nov. and Pseudaminobacter defluvii sp. nov. Int J Syst Bacteriol 49, 887–897.[CrossRef]
    [Google Scholar]
  12. Kämpfer, P., Dreyer, U., Neef, A., Dott, W. & Busse, H.-J. ( 2003; ). Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53, 93–97.[CrossRef]
    [Google Scholar]
  13. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). MEGA2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  14. Lapage, S. P., Sneath, P. H. A., Lessel, E. F., Skerman, V. B. D., Seeliger, H. P. R. & Clark, W. A. (editors) ( 1992; ). International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code. Washington, DC: American Society for Microbiology.
  15. Lebuhn, M., Achouak, W., Schloter, M., Berge, O., Meier, H., Barakat, M., Hartmann, A. & Heulin, T. ( 2000; ). Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov. Int J Syst Evol Microbiol 50, 2207–2223.[CrossRef]
    [Google Scholar]
  16. Lechner, U., Baumbach, R., Becker, D., Kitunen, V., Auling, G. & Salkinoja-Salonen, M. ( 1995; ). Degradation of 4-chloro-2-methylphenol by an activated sludge isolate and its taxonomic description. Biodegradation 6, 83–92.[CrossRef]
    [Google Scholar]
  17. Parte, A. ( 2002; ). IJSEM at SGM, 5 years on. Int J Syst Evol Microbiol 52, 291–292.
    [Google Scholar]
  18. Stackebrandt, E., Frederiksen, W., Garrity, G. M. & 10 other authors ( 2002; ). Report of the ad-hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  19. Strunk, O., Gross, O. & Reichel, B. & 10 other authors ( 2000; ). arb: a software environment for sequence data (http://www.mikro.biologie.tu-muenchen.de). Department of Microbiology, Technische Universität München, Munich, Germany.
  20. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24, 4876–4882.
    [Google Scholar]
  21. Tindall, B. J. ( 1990; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef]
    [Google Scholar]
  22. Velasco, J., Romero, C., López-Goñi, I., Leiva, J., Díaz, R. & Moriyón, I. ( 1998; ). Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp. Int J Syst Bacteriol 48, 759–768.[CrossRef]
    [Google Scholar]
  23. Ventosa, A., Marquez, M. C., Kocur, M. & Tindall, B. J. ( 1993; ). Comparative study of “Micrococcus” sp. strains CCM 168 and CCM 1405 and members of the genus Salinicoccus. Int J Syst Bacteriol 43, 245–248.[CrossRef]
    [Google Scholar]
  24. Yokota, A., Akagawa-Matsushita, M., Hiraishi, A., Katayama, Y., Urakami, T. & Yamasato, K. ( 1992; ). Distribution of quinone systems in microorganisms: Gram-negative eubacteria. Bull Jpn Fed Cult Coll 8, 136–171.
    [Google Scholar]
  25. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R. ( 1998; ). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef]
    [Google Scholar]
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vol. , part 3, pp. 893 - 896

Two-dimensional thin-layer chromatogram of polar lipids of strain ISO 196 . Phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phosphatidylcholine (PC), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), unknown phospholipid (PL), unknown aminolipids (AL1, AL2), unknown aminophospholipid (APL), unknown lipids only stained by molybdatophosphoric acid (L1, L2, L3).



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