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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 53, Issue 6

sp. nov., a novel species that actively solubilizes phosphate Free

Abstract

A bacterial strain (designated IH5), isolated from rhizospheric soil of grasses growing spontaneously in Spanish soil, actively solubilized phosphates when bicalcium phosphate was used as a phosphorus source. This strain was Gram-negative, strictly aerobic, rod-shaped and motile. The strain produced catalase, but not oxidase. Cellulose, casein, starch, gelatin, aesculin and urea were not hydrolysed. Growth was observed with many carbohydrates as the carbon source. The main non-polar fatty acids detected were hexadecenoic acid (C), hexadecanoic acid (C) and octadecenoic acid (C). The hydroxy fatty acids detected were 3-hydroxydecanoic acid (C 3-OH), 3-hydroxydodecanoic acid (C 3-OH) and 2-hydroxydodecanoic acid (C 2-OH). Phylogenetic analysis of 16S rRNA indicated that this bacterium belongs to the genus in the -subclass of the and that the closest related species is . The DNA G+C content was 61 mol%. DNA–DNA hybridization showed 23 % relatedness between strain IH5 and DSM 11363. Therefore, strain IH5 belongs to a novel species from the genus , for which the name sp. nov. is proposed (type strain, IH5=LMG 21640=CECT 5726).

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2003-11-01
2025-03-20
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Pseudomonas rhizosphaerae sp. nov., a novel species that actively solubilizes phosphate in vitro
Int J Syst Evol Microbiol 53, 2067 (2003); https://doi.org/10.1099/ijs.0.02703-0
/content/journal/ijsem/10.1099/ijs.0.02703-0
/content/journal/ijsem/10.1099/ijs.0.02703-0
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vol. , part 6, pp. 2067 – 2072

Electron micrograph of strain IH5 .

Wider phylogenetic tree showing the placement of within the genus .

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