1887

Abstract

A novel anaerobic, thermophilic, xylanolytic, motile rod-shaped bacterium with a sheath-like outer structure (toga) was isolated from a Mexican oil well in the Gulf of Mexico. Strain MET12 was a Gram-negative bacterium, reducing elemental sulfur, thiosulfate and sulfite to hydrogen sulfide. Its optimum growth conditions were 55 °C, pH 6·6, 3 % NaCl and 0·15 % MgCl.6HO. The DNA G+C content was 36·1 mol%. Phylogenetically, strain MET12 was related to members of genus , with similarities to , , and varying from 97·6 to 98·8 %. However DNA–DNA relatedness values between these species and strain MET12 were lower than 70 %. As strain MET12 (=DSM 14811=CIP 107371) was genomically and phenotypically different from existing species, it is proposed as the type strain of a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02702-0
2004-01-01
2020-01-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/1/ijs540169.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02702-0&mimeType=html&fmt=ahah

References

  1. Alain, K., Marteinsson, V. T., Miroshnichenko, M. L., Bonch-Osmolovskaya, E. A., Prieur, D. & Birrien, J.-L. ( 2002; ). Marinitoga piezophila sp. nov., a rod-shaped, thermo-piezophilic bacterium isolated under high hydrostatic pressure from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 52, 1331–1339.[CrossRef]
    [Google Scholar]
  2. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  3. Balch, W. E., Fox, G. E., Magrum, R. J., Woese, C. R. & Wolfe, R. S. ( 1979; ). Methanogens: reevaluation of a unique biological group. Microbiol Rev 43, 260–296.
    [Google Scholar]
  4. Benson, D. A., Boguski, M. S., Lipman, D. J., Oullette, B. F. F., Rapp, B. A. & Wheeler, D. L. ( 1999; ). GenBank. Nucleic Acids Res 27, 12–17.[CrossRef]
    [Google Scholar]
  5. Cord-Ruwisch, R. ( 1985; ). A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 4, 33–36.[CrossRef]
    [Google Scholar]
  6. Davey, M. E., Wood, W. A., Key, R., Nakamura, K. & Stahl, D. A. ( 1993; ). Isolation of three species of Geotoga and Petrotoga: two new genera, representing a new lineage in the bacterial line of descent distantly related to the “Thermotogales”. Syst Appl Microbiol 16, 191–200.[CrossRef]
    [Google Scholar]
  7. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  8. Escara, J. F. & Hutton, J. R. ( 1980; ). Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions; acceleration of the renaturation rate. Biopolymers 19, 1315–1327.[CrossRef]
    [Google Scholar]
  9. Fardeau, M.-L., Cayol, J.-L., Magot, M. & Ollivier, B. ( 1993; ). H2 oxidation in the presence of thiosulfate by a Thermoanaerobacter strain isolated from an oil-producing well. FEMS Microbiol Lett 113, 327–332.[CrossRef]
    [Google Scholar]
  10. Fardeau, M.-L., Ollivier, B., Patel, B. K. C., Magot, M., Thomas, P., Rimbault, A., Rocchiccioli, F. & Garcia, J.-L. ( 1997a; ). Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. Int J Syst Bacteriol 47, 1013–1019.[CrossRef]
    [Google Scholar]
  11. Fardeau, M.-L., Patel, B. K. C., Magot, M. & Ollivier, B. ( 1997b; ). Utilization of serine, leucine, isoleucine, and valine by Thermoanaerobacter brockii in the presence of thiosulfate or Methanobacterium sp. as electron acceptors. Anaerobe 3, 405–410.[CrossRef]
    [Google Scholar]
  12. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  13. Grassia, G. S., McLean, K. M., Glénat, P., Bauld, J. & Sheehy, A. J. ( 1996; ). A systematic survey for thermophilic fermentative bacteria and archaea in high temperature petroleum reservoirs. FEMS Microbiol Ecol 21, 47–58.[CrossRef]
    [Google Scholar]
  14. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98 NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  15. Hungate, R. E. ( 1969; ). A roll-tube method for the cultivation of strict anaerobes. Methods Microbiol 3B, 117–132.
    [Google Scholar]
  16. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  17. Jahnke, K. D. ( 1992; ). Basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef]
    [Google Scholar]
  18. Jeanthon, C., Reysenbach, A. L., L'Haridon, S., Gambacorta, A., Pace, N. R., Glénat, P. & Prieur, D. ( 1995; ). Thermotoga subterranea sp. nov., a new thermophilic bacterium isolated from a continental oil reservoir. Arch Microbiol 164, 91–97.[CrossRef]
    [Google Scholar]
  19. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 211–232. Edited by H. N. Munro. New York: Academic Press.
  20. Koussémon, M., Combet-Blanc, Y., Patel, B. K. C., Cayol, J.-L., Thomas, P., Garcia, J.-L. & Ollivier, B. ( 2001; ). Propionibacterium microaerophilum sp. nov., a microaerophilic bacterium isolated from olive mill wastewater. Int J Syst Evol Microbiol 51, 1373–1382.
    [Google Scholar]
  21. L'Haridon, S., Miroshnichenko, M. L., Hippe, H., Fardeau, M.-L., Bonch-Osmolovskaya, E., Stackebrandt, E. & Jeanthon, C. ( 2001; ). Thermosipho geolei sp. nov., a thermophilic bacterium isolated from a continental petroleum reservoir in Western Siberia. Int J Syst Evol Microbiol 51, 1327–1334.
    [Google Scholar]
  22. L'Haridon, S., Miroshnichenko, M. L., Hippe, H., Fardeau, M.-L., Bonch-Osmolovskaya, E. A., Stackebrandt, E. & Jeanthon, C. ( 2002; ). Petrotoga olearia sp. nov. and Petrotoga sibirica sp. nov., two thermophilic bacteria isolated from a continental petroleum reservoir in Western Siberia. Int J Syst Evol Microbiol 52, 1715–1722.[CrossRef]
    [Google Scholar]
  23. Lien, T., Madsen, M., Rainey, F. A. & Birkeland, N.-K. ( 1998; ). Petrotoga mobilis sp. nov., from a North Sea oil-production well. Int J Syst Bacteriol 48, 1007–1013.[CrossRef]
    [Google Scholar]
  24. Magot, M., Ollivier, B. & Patel, B. K. C. ( 2000; ). Microbiology of petroleum reservoirs. Antonie van Leeuwenhoek 77, 103–116.[CrossRef]
    [Google Scholar]
  25. Maidak, B. L., Cole, J. R., Lilburn, T. G. & 7 other authors ( 2001; ). The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29, 173–174.[CrossRef]
    [Google Scholar]
  26. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  27. Miller, G. L. ( 1959; ). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31, 426–428.[CrossRef]
    [Google Scholar]
  28. Ravot, G., Magot, M., Fardeau, M.-L., Patel, B. K. C., Prensier, G., Egan, A., García, J.-L. & Ollivier, B. ( 1995a; ). Thermotoga elfii sp. nov., a novel thermophilic bacterium from an African oil-producing well. Int J Syst Bacteriol 45, 308–314.[CrossRef]
    [Google Scholar]
  29. Ravot, G., Ollivier, B., Magot, M., Patel, B. K. C., Crolet, J.-L., Fardeau, M.-L. & Garcia, J.-L. ( 1995b; ). Thiosulfate reduction, an important physiological feature shared by members of the order Thermotogales. Appl Environ Microbiol 61, 2053–2055.
    [Google Scholar]
  30. Ravot, G., Ollivier, B., Fardeau, M.-L., Patel, B. K. C., Andrews, K. T., Magot, M. & Garcia, J.-L. ( 1996; ). l-Alanine production from glucose fermentation by hyperthermophilic members of the domains Bacteria and Archaea: a remnant of an ancestral metabolism? Appl Environ Microbiol 62, 2657–2659.
    [Google Scholar]
  31. Reysenbach, A.-L. ( 2001; ). Order I. Thermotogales ord. nov. Huber and Stetter 1992c, 3809. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, p. 369. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  32. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  33. Stetter, K. O., Huber, R., Blöchl, E., Kurr, M., Eden, R. D., Fielder, M., Cash, H. & Vance, I. ( 1993; ). Hyperthermophilic archaea are thriving in deep North Sea and Alaskan oil reservoirs. Nature 365, 743–745.[CrossRef]
    [Google Scholar]
  34. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  35. Wery, N., Lesongeur, F., Pignet, P., Derennes, V., Cambon-Bonavita, M.-A., Godfroy, A. & Barbier, G. ( 2001; ). Marinitoga camini gen. nov., sp. nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 495–504.
    [Google Scholar]
  36. Winker, S. & Woese, C. R. ( 1991; ). A definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA characteristics. Syst Appl Microbiol 13, 161–165.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02702-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02702-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error