1887

Abstract

Two thermophilic, Gram-negative, non-spore-forming, multicellular filamentous micro-organisms were isolated from thermophilic granular sludge in an upflow anaerobic sludge blanket reactor treating fried soybean-curd manufacturing waste water (strain UNI-1) and from a hot spring sulfur-turf in Japan (strain STL-6-O1). The filaments were longer than 100 μm and of 0·2–0·3 μm (strain UNI-1) or 0·7–0·8 μm (strain STL-6-O1) in width. Strain UNI-1 was a strictly anaerobic organism. The optimum temperature for growth was around 55 °C; growth occurred in the range 50–60 °C. The optimum pH for growth was around 7·0; growth occurred in the range pH 6·0–8·0. Strain STL-6-O1 was a facultatively aerobic bacterium. The optimum temperature for growth was around 55 °C; growth occurred in the range 37–65 °C. The optimum pH for growth was around 7·5–8·0; growth occurred in the range pH 7·0–9·0. The two organisms grew chemo-organotrophically on a number of carbohydrates and amino acids in the presence of yeast extract. The G+C content of the DNA of strains UNI-1 and STL-6-O1 was 54·5 and 59·0 mol%, respectively. Major cellular fatty acids for strain UNI-1 were C, C, C and C, whereas those for strain STL-6-O1 were C, C, C and iso-C. MK-10 was the major quinone from aerobically grown STL-6-O1 cells. Phylogenetic analyses based on 16S rDNA sequences revealed that both strains belong to an uncultured, previously recognized clone lineage of the phylum (formerly known as green non-sulfur bacteria). These phenotypic and genetic properties suggested that each strain should be classified into a new independent genus; hence, the names and are proposed for strains UNI-1 (=JCM 11387=DSM 14523) and STL-6-O1(=JCM 11388=DSM 14525), respectively. These strains represent the type and sole species of the genera and , respectively.

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2003-11-01
2019-10-17
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vol. , part 6, pp. 1843-1851

A phylogenetic tree (constructed using a large dataset) showing the relationships of UNI-1T and STL-6-O1T with members of the phylum Chloroflexi is available when you click here. (PDF format)



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