1887

Abstract

Elm yellows group (16SrV) phytoplasmas, which are associated with devastating diseases in elm, grapevine, blackberry, cherry, peach and several other plant species in America, Europe and Asia, represent one of the most diverse phytoplasma clusters. On the basis of phylogenetic analysis of 16S rDNA sequences, elm yellows group phytoplasmas form a discrete subclade within the phytoplasma clade. Three phylogenetic parameters, namely 16S rRNA, ribosomal protein and genes, have been evaluated for their usefulness in differentiating elm yellows group phytoplasmas. RFLP analysis of 16S rRNA sequences differentiated the elm yellows group phytoplasmas into five subgroups. Twelve RFLP subgroups were differentiated on the basis of ribosomal protein and 13 were differentiated using gene sequences. Phylogenetic analysis of the ribosomal protein genes and gene alone or in combination indicated that the subgroups constitute 12 genetically distinct lineages, each of which appears to have evolved under different ecological constraints such as specific vector or plant hosts. On the basis of unique DNA and biological properties, it is proposed that the elm yellows phytoplasma EY1 represents a novel taxon, ‘ Phytoplasma ulmi’.

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2004-03-01
2019-12-06
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Analysis of putative restriction sites in ribosomal protein operon sequences (1.2 kb, contains and genes), amplified by nested PCR with primer pair rp(V)F1/rpR1 followed by primer pair rp(V)F1A/rp(V)R1A from representative phytoplasma strains in the elm yellows phytoplasma group (16SrV). Maps were generated using the MapDraw option of the DNASTAR program and aligned manually for comparison of restriction sites. Asterisks indicate putative restriction sites that differentiate the representative strains in the elm yellows phytoplasma group (16SrV). Strain abbreviations are defined in Table 1. [PDF](115 KB)

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Analysis of putative restriction sites in the gene sequence (1.2 kb). The sequence was trimmed from a DNA fragment (1.4 kb) amplified by PCR with primer pair FD9f/r from representative phytoplasma strains in the elm yellows phytoplasma group (16SrV). Maps were generated using the MapDraw option of the DNASTAR program and aligned manually for comparison of restriction sites. Asterisks indicate putative restriction sites that differentiate the representative strains in the elm yellows phytoplasma group (16SrV). Strain abbreviations are defined in Table 1. [PDF](111 KB)

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