1887

Abstract

A novel mesophilic, facultatively anaerobic, sulfur-oxidizing bacterial strain, designated gps61, was isolated from a surface rock sample collected from the hydrothermal field of Suiyo Seamount on the Izu-Bonin Arc in the Western Pacific Ocean. Cells of the isolate were rod-shaped with a single sheathed polar flagellum. Neither extensive internal membranes nor storage materials were present in the cells. In a 20 % CO atmosphere, strain gps61 grew using thiosulfate, sulfur or tetrathionate as electron donors and oxygen or nitrate as electron acceptors. Other substrates, including organic acids and sugars, did not support growth, indicating that strain gps61 was an obligate chemolithoautotroph. 16S rRNA gene sequence analysis revealed that strain gps61 was closely related to 106 (98.5 % sequence similarity) in the order . Phylogenetic trees grouped strain gps61 and in the same cluster along with and , but it was apparent from the analysis that the novel strain had definitely departed from the family lineage. On the basis of its phylogenetic position along with its morphological and physiological characteristics, strain gps61 ( = NBRC 101261  = DSM 18546) represents a novel species of the genus , for which the name sp. nov. is proposed. In addition, we propose a novel family name, , in the order , to accommodate the genera , and .

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2011-10-01
2019-08-26
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Time-courses of the growth of strain gps61 and concentrations of thiosulfate and sulfate in the presence of (a) 20 mM thiosulfate or (b) 1% elemental sulfur as an electron donor. [PDF](31KB)

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Phylogenetic relationships based on 16S rRNA gene sequences of strain gps61 , type species of and , and environmental clones, inferred from the neighbour-joining method after alignment with the ARB program. [PDF](29KB)

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