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Abstract

Bacterial strain G72 was isolated from surface-sterilized roots of growing in its natural habitat. This Gram-positive, rod-shaped, non-motile, microaerophilic and aerobically growing isolate was characterized by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, strain G72 belongs to the genus . However, reassociation values in a DNA–DNA hybridization analysis with closely related strains were between 45.1  and 15.9 %. The DNA GC content was 70.1 mol%. Strain G72 possessed a peptidoglycan type based on B2β with partial substitution of glutamic acid by 3-hydroxy glutamic acid (Glu/Hyg–Gly–-Orn). The isolate contained MK-13, MK-12 and MK-11 as major respiratory quinones and anteiso-C, anteiso-C and iso-C as predominant fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids. Based on its physiological and biochemical traits, as well as genotypic results, strain G72 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is G72 ( = DSM 23203  = LMG 25521).

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2012-04-01
2019-09-17
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