%0 Journal Article %A Zhang, Xiaoxia %A Li, Baoming %A Wang, Haisheng %A Sui, Xinhua %A Ma, Xiaotong %A Hong, Qing %A Jiang, Ruibo %T Rhizobiumpetrolearium sp. nov., isolated from oil-contaminated soil %D 2012 %J International Journal of Systematic and Evolutionary Microbiology, %V 62 %N Pt_8 %P 1871-1876 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.026880-0 %I Microbiology Society, %X Two Gram-negative, aerobic, rod-shaped bacteria, designated strains SL-1T and F11, which had the ability to decompose polycyclic aromatic hydrocarbons (PAHs), were isolated from soil samples contaminated by oil. The cells were motile by polar or lateral flagella. According to comparison of 16S rRNA gene sequences, strains SL-1T and F11 were identical and showed the greatest degree of similarity (96.8 %) to both Rhizobium oryzae Alt505T and Rhizobium mesosinicum CCBAU 25010T; however, only Rhizobium oryzae with SL-1T and F11 formed a separate clade. There were low similarities (<90 %) between the atpD and recA sequences of the two strains and those of the genus of Rhizobium . The bacteria grew at temperatures of 10–40 °C with an optimum of 30 °C. The pH range for growth was 6.0–10.0 and optimum pH was 7.0–8.0. Growth occurred at NaCl concentrations up to 3.0 % (w/v). They were catalase- and oxidase-positive. The main cellular fatty acids were summed feature 8 (18 : 1ω7c and/or 18 : 1ω6c) and 16 : 0. The DNA G+C content was 62.2 mol%. Strain SL-1T showed 29 and 0 % DNA–DNA relatedness, respectively, with the most related strains R. oryzae Alt505T and R. mesosinicum CCBAU 25010T according to phylogenic analysis of the 16S rRNA gene. According to physiological and biochemical characteristics and genotypic data obtained in this work, the bacteria represent a novel species of the genus Rhizobium , and the name Rhizobium petrolearium is proposed. The type strain is SL-1T ( = ACCC 11238T = KCTC 23288T) and it could nodulate Medicago sativa in nodulation tests. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.026880-0