1887

Abstract

A facultatively psychrophilic bacterium, strain MD17, which hydrolyses lipids at 5 °C, was isolated from the Monbetsu coast of the Okhotsk Sea in Hokkaido, Japan, when ice carried by the cold current came to the area. The isolate is an aerobic, non-motile coccobacillus that reduces nitrate to nitrite and hydrolyses Tweens 20, 40, 60 and 80, but not gelatin, DNA or alginic acid. The isolate grows at 0 °C, but not at temperatures higher than 36 °C; its optimum growth temperature is 25 °C. It grows in the presence of 0–10 % NaCl. Its major isoprenoid quinone is ubiquinone-8 (Q-8) and its DNA G+C content is 46·7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MD17 is closely related to DSM 12194 (99·0 % similarity) and DSM 7229 (98·7 % similarity). DNA–DNA hybridization revealed 45·9 % relatedness between strain MD17 and ATCC 43116 and 33·4 % between strain MD17 and ATCC 700754. Based on physiological and biochemical characteristics, phylogenetic position (as determined by 16S rRNA gene sequence analysis) and DNA–DNA relatedness, it is concluded that the isolate should be designated as a novel species, for which the name sp. nov. is proposed. The type strain is MD17 (=NCIMB 13931=JCM 11840).

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2003-11-01
2019-10-13
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DNA–DNA relatedness among strains examined in this study [PDF](169 KB)

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Transmission electron micrograph of a negatively stained cell of MD17 , showing non-flagellated and coccobacillar morphology. Bar, 1 µm.

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Transmission electron micrograph of an ultra-thin section of MD17 . Bar, 1 µm.

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Scanning electron micrograph of cells of platinum/palladium-coated MD17 . Bar, 1 µm.

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