1887

Abstract

Eight strains of Gram-positive, catalase- and oxidase-positive cocci were isolated from the skin of llamas ( L.) and characterized using a polyphasic approach. These strains were assigned to the genus on the basis of their phenotypic properties (resistance to bacitracin and sensitivity to furazolidone) and DNA base content (40–42 mol% G+C). Phylogenetic analysis based on 16S rDNA confirmed that the strains are members of the genus . They differed from all hitherto described macrococcal species in their production of phosphatase and reduction of nitrate (most strains) and the inability to produce acid from glycerol or to grow in 7·5 % NaCl. Ribotyping (RI), macrorestriction analysis (I) and fatty acid methyl ester analysis divided the strains from llamas into three stable clusters. Moreover, ribotyping differentiated the strains analysed not only from previously described macrococcal species but also from oxidase-positive staphylococci. DNA–DNA hybridization confirmed that the three clusters represent separate genomic groups (similarity values<54 %). All the results showed that the strains represent three novel species, for which the names sp. nov. (type strain CCM 4809=LMG 21711), sp. nov. (type strain CCM 4811=LMG 21712) and sp. nov. (type strain CCM 4815=LMG 21713) are proposed.

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2003-09-01
2020-01-19
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