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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 61, Issue 12

sp. nov. and sp. nov., isolated from rhizosphere soil of Chinese cabbage () Free

Abstract

Two bacterial strains, 01-96 and 15-51, isolated from rhizosphere soil of Chinese cabbage () were characterized by using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain 01-96 was phylogenetically related most closely to 15-52 and CL-GP80 (96.7 and 96.7 % similarity, respectively), and strain 15-51 to G-1 and 15-52 (97.2 and 97.1 %, respectively). However, levels of DNA–DNA relatedness between strain 15-51 and KACC 14287 and KACC 11317 were low (<50 %). The G+C content of the genomic DNA was 37.8 mol% for strain 01-96 and 38.6 mol% for strain 15-51. The major fatty acids of the two strains were iso-C 3-OH, iso-C and summed feature 3 (iso-C 2-OH and/or Cω7). The results of DNA–DNA hybridization and phenotypic data showed that strains 01-96 and 15-51 could be distinguished from their closest phylogenetic relatives, and that these strains represented two novel species of the genus , for which the names sp. nov. (type strain 01-96  = KACC 14938  = NBRC 107690) and sp. nov. (type strain 15-51  = KACC 14939  = NBRC 107691) are proposed.

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Funding
This study was supported by the:
  • National Academy of Agricultural Science (Award 200901FHT020609339)
© 2011 IUMS
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2011-12-01
2024-04-16
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Pedobacter rhizosphaerae sp. nov. and Pedobacter soli sp. nov., isolated from rhizosphere soil of Chinese cabbage (Brassica campestris)
Int J Syst Evol Microbiol 61, 2874 (2011); https://doi.org/10.1099/ijs.0.026781-0
/content/journal/ijsem/10.1099/ijs.0.026781-0
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