1887

Abstract

Two bacterial strains, 01-96 and 15-51, isolated from rhizosphere soil of Chinese cabbage () were characterized by using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain 01-96 was phylogenetically related most closely to 15-52 and CL-GP80 (96.7 and 96.7 % similarity, respectively), and strain 15-51 to G-1 and 15-52 (97.2 and 97.1 %, respectively). However, levels of DNA–DNA relatedness between strain 15-51 and KACC 14287 and KACC 11317 were low (<50 %). The G+C content of the genomic DNA was 37.8 mol% for strain 01-96 and 38.6 mol% for strain 15-51. The major fatty acids of the two strains were iso-C 3-OH, iso-C and summed feature 3 (iso-C 2-OH and/or Cω7). The results of DNA–DNA hybridization and phenotypic data showed that strains 01-96 and 15-51 could be distinguished from their closest phylogenetic relatives, and that these strains represented two novel species of the genus , for which the names sp. nov. (type strain 01-96  = KACC 14938  = NBRC 107690) and sp. nov. (type strain 15-51  = KACC 14939  = NBRC 107691) are proposed.

Funding
This study was supported by the:
  • , National Academy of Agricultural Science , (Award 200901FHT020609339)
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2011-12-01
2020-08-10
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