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Abstract

Several bacterial strains were isolated from root nodules of plants grown in a soil from Portugal. The strains were Gram-negative, aerobic, curved rod-shaped and motile. The isolates were catalase- and oxidase-positive. The TP-RAPD (two-primer randomly amplified polymorphic DNA) patterns of all strains were identical, suggesting that they belong to the same species. The complete 16S rDNA sequence of a representative strain was obtained and phylogenetic analysis based on the neighbour-joining method indicated that this bacterium belongs to the - and that the closest related genus is . The DNA G+C content ranged from 57·9 to 61·9 mol%. Growth was observed with many different carbohydrates and organic acids including caprate, malate, citrate and phenylacetate. No growth was observed with maltose, -inositol, -erythritol or adipate as sole carbon source. According to the phenotypic and genotypic data obtained in this work, the bacterium represents a novel species of the genus , and the name sp. nov. is proposed. The type strain is P6-12 (=LMG 21710=CECT 5661).

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2003-11-01
2019-10-16
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Transmission electron micrographs of strain P6-12 grown in nutrient agar for 48 h showing one (a) and two (b) polar flagella. Bars, 0.15 µm.

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Phylogenetic analysis based on 16S rRNA gene sequences available from GenBank (accession numbers in parentheses) constructed after multiple alignment of data by CLUSTAL W software, showing the position of strain P6-12 . Distances were calculated according to Kimura's two-parameter method and clustering with neighbour-joining method was performed by using the software package MEGA 2.1, as described in Methods. Bootstrap values based on 1000 replications are listed as percentages at branching points. The tree was rooted using ATCC 11996 as the outgroup. Bar, 1 substitution per 100 nt. [PDF](105 KB)

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RFLP patterns after digestion with the restriction endonucleases I and I (Amersham-Pharmacia Biotech) of 16SrDNA of the type strains of previously described species of and of strains isolated in this study (represented by strain P6-12 ). Lanes: 1 and 5, DSM 6445 ; 2 and 6, DSM 9440 ; 3 and 7, DSM 13128 ; 4 and 8, strain P6-12 ; MW, 100 bp ladder (Pharmacia).

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TP-RAPD patterns of the type strains of previously described species of and of strains isolated in this study (represented by strain P6-12 ). Lanes: 1, DSM 6445 ; 2, DSM 9440 ; 3, DSM 13128 ; 4, strain P6-12 ; MW, MassRuler molecular mass marker (Fermentas).

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