1887

Abstract

Several bacterial strains were isolated from root nodules of plants grown in a soil from Portugal. The strains were Gram-negative, aerobic, curved rod-shaped and motile. The isolates were catalase- and oxidase-positive. The TP-RAPD (two-primer randomly amplified polymorphic DNA) patterns of all strains were identical, suggesting that they belong to the same species. The complete 16S rDNA sequence of a representative strain was obtained and phylogenetic analysis based on the neighbour-joining method indicated that this bacterium belongs to the - and that the closest related genus is . The DNA G+C content ranged from 57·9 to 61·9 mol%. Growth was observed with many different carbohydrates and organic acids including caprate, malate, citrate and phenylacetate. No growth was observed with maltose, -inositol, -erythritol or adipate as sole carbon source. According to the phenotypic and genotypic data obtained in this work, the bacterium represents a novel species of the genus , and the name sp. nov. is proposed. The type strain is P6-12 (=LMG 21710=CECT 5661).

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2003-11-01
2021-04-16
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References

  1. Arahal D. R., García M. T., Ludwig W., Schleifer K. H., Ventosa A. 2001; Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. Int J Syst Evol Microbiol 51:1443–1448
    [Google Scholar]
  2. Baldani J. I., Baldani V. L. D., Seldin L., Döbereiner J. 1986; Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium. Int J Syst Bacteriol 36:86–93 [CrossRef]
    [Google Scholar]
  3. Baldani J. I., Pot B., Kirchhof G. 8 other authors 1996; Emended description of Herbaspirillum ; inclusion of [ Pseudomonas ] rubrisubalbicans , a milk plant pathogen, as Herbaspirillum rubrisubalbicans comb. nov.; and classification of a group of clinical isolates (EF group 1) as Herbaspirillum species 3. Int J Syst Bacteriol 46:802–810 [CrossRef]
    [Google Scholar]
  4. Bergersen F. J. 1961; The growth of Rhizobium in synthetic media. Aust J Biol 14:349–360
    [Google Scholar]
  5. Döbereiner J. 1995; Isolation and identification of aerobic nitrogen-fixing bacteria from soil and plants. In Methods in Applied Soil Microbiology and Biochemistry pp 134–141Edited by Alef K., Nannipieri P. London: Academic Press;
    [Google Scholar]
  6. Döbereiner J., Reis V. M., Paula M. A., Olivares F. L. 1993; Endophytic diazotrophs in sugar cane, tuber plants and cereals. In New Horizons in Nitrogen Fixation pp 671–676Edited by Palacios R., Mora J., Newton W. E. Dordrecht: Kluwer;
    [Google Scholar]
  7. Doetsch R. N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp 21–33Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Elbeltagy A., Nishioka K., Suzuki H., Sato T., Sato Y.-I., Morisaki H., Mitsui H., Minamisawa K. 2000; Isolation and characterization of endophytic bacteria from wild and traditionally cultivated rice varieties. Soil Sci Plant Nutr 46:617–629 [CrossRef]
    [Google Scholar]
  9. Elbeltagy A., Nishioka K., Sato T., Suzuki H., Ye B., Hamada T., Isawa T., Mitsui H., Minamisawa K. 2001; Endophytic colonization and in planta nitrogen fixation by a Herbaspirillum sp. isolated from wild rice species. Appl Environ Microbiol 67:5285–5293 [CrossRef]
    [Google Scholar]
  10. Gillis M., Döbereiner J., Pot B., Goor M., Falsen E., Hoste B., Reinhold B., Kersters K. 1990; Taxonomic relationships between [ Pseudomonas ] rubrisubalbicans , some clinical isolates (EF group 1), Herbaspirillum seropedicae and [ Aquaspirillum ] autotrophicum . In Nitrogen Fixation Associated with Non-legumes pp 293–294Edited by Polsinelli M., Materassi R., Vincenzini M. Dordrecht: Kluwer;
    [Google Scholar]
  11. James E. K., Olivares F. L., Baldani J. I., Döbereiner J. 1997; Herbaspirillum , an endophytic diazotroph colonizing vascular tissue in leaves of Sorghum bicolor L. Moench. J Exp Bot 48:785–797 [CrossRef]
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  13. Kirchhof G., Eckert B., Stoffels M., Baldani J. I., Reis V. M., Hartmann A. 2001; Herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants. Int J Syst Evol Microbiol 51:157–168
    [Google Scholar]
  14. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001 Molecular Evolutionary Genetics Analysis Software Tempe, AZ: Arizona State University;
    [Google Scholar]
  15. Olivares F. L., Baldani V. L. D., Reis V. M., Baldani J. I., Döbereiner J. 1996; Occurrence of the endophytic diazotrophs Herbaspirillum spp. in roots, stems, and leaves, predominantly of Gramineae. Biol Fertil Soils 21:197–200 [CrossRef]
    [Google Scholar]
  16. Olivares F. L., James E. K., Baldani J. I., Döbereiner J. 1997; Infection of mottled stripe disease-susceptible and resistant sugar cane varieties by the endophytic diazotroph Herbaspirillum . New Phytol 135:723–737 [CrossRef]
    [Google Scholar]
  17. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
    [Google Scholar]
  18. Reinhold-Hurek B., Hurek T. 1998; Life in grasses: diazotrophic endophytes. Trends Microbiol 6:139–144 [CrossRef]
    [Google Scholar]
  19. Rivas R., Velázquez E., Valverde A., Mateos P. F., Martínez-Molina E. 2001; A two primers random amplified polymorphic DNA procedure to obtain polymerase chain reaction fingerprints of bacterial species. Electrophoresis 22:1086–1089 [CrossRef]
    [Google Scholar]
  20. Rivas R., Velázquez E., Willems A., Vizcaíno N., Subba-Rao N. S., Mateos P. F., Gillis M., Dazzo F. B., Martínez-Molina E. 2002; A new species of Devosia that forms a unique nitrogen-fixing root-nodule symbiosis with the aquatic legume Neptunia natans (L. f.) Druce. Appl Environ Microbiol 68:5217–5222 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Stoltzfus J. R., So R., Malarvithi P. P., Ladha J. K., de Bruijn F. J. 1997; Isolation of endophytic bacteria from rice and assessment of their potential for supplying rice with biologically fixed nitrogen. Plant Soil 194:25–36 [CrossRef]
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  24. Vincent J. M. 1970; The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of Root-Nodule Bacteria pp 1–13Edited by Vincent J. M. Oxford: Blackwell Scientific;
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
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