1887

Abstract

A novel endophytic actinobacterium, designated strain EUM 378, was isolated from the surface-sterilized root tissue of , a eucalyptus tree known as Grey Box. Phylogenetic evaluation based on 16S rRNA gene sequence analysis, including alignment with taxon-specific 16S rRNA gene signature nucleotides, placed this isolate as a member of the family . Strain EUM 378 showed >5.5 % 16S rRNA gene sequence divergence from other members of this family and was related most closely to YIM 48868 (94.2 %) and IM 7744 (94.4 %). This Gram-positive, aerobic actinobacterium has well-developed substrate mycelia that fragment into small rods. Chemotaxonomic data revealed that the cell wall contains -diaminopimelic acid, ribose, glucose and rhamnose. MK-10(H) is the predominant menaquinone. Chemotaxonomic and phylogenetic evidence confirmed that strain EUM 378 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is EUM 378 ( = DSM 45355 = ACM 5289).

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2011-09-01
2020-01-24
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vol. , part 9, pp. 2135–2140

Whole-cell fatty acid composition of EUM 378 and reference strains

Cultural characteristics of strain EUM 378 after 21 days growth

Scanning electron micrograph of strain EUM 378 grown on HPDA for 21 days at 27 °C

Maximum-parsimony tree showing the relationship between and selected micro-organisms belonging to the family

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