1887

Abstract

Three strains were isolated from cultured aquatic organisms. They were Gram-negative, oxidase-positive, motile, fermentative, arginine dihydrolase-positive, lysine and ornithine decarboxylase-negative and sensitive to vibriostatic agent O/129. These strains differ from other related species by several phenotypic features, which include acetoin and indole production and utilization of amygdalin and -mannitol. Comparison of 16S rDNA sequences showed a close relationship to the recently described species (96·6 %) and (96·4 %) and to (96·6 %), but DNA–DNA hybridization experiments showed that the three isolates form a tight novel species with ⩽30 % DNA–DNA similarity to its closest phylogenetic neighbours. sp. nov. is proposed, with LMG 19999 (=CAIM 530=STD3-1057; DNA G+C content, 44·9 mol%) as the type strain.

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2003-09-01
2024-12-05
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References

  1. Alsina M., Blanch A. R. 1994a; A set of keys for biochemical identification of environmental Vibrio species. J Appl Bacteriol 76:79–85 [CrossRef]
    [Google Scholar]
  2. Alsina M., Blanch A. R. 1994b; Improvement and update of a set of keys for biochemical identification of Vibrio species. J Appl Bacteriol 77:719–721 [CrossRef]
    [Google Scholar]
  3. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  4. Austin B. 1988 Marine Microbiology Cambridge: Cambridge University Press;
    [Google Scholar]
  5. Austin B., Lee J. V. 1992; Aeromonadaceae and Vibrionaceae . In Identification Methods in Applied and Environmental Microbiology pp 163–182Edited by Board R. G., Jones D., Skinner F. A. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  6. Austin B., Alsina M., Austin D. A.9 other authors 1995; Identification and typing of Vibrio anguillarum : a comparison of different methods. Syst Appl Microbiol 18:285–302 [CrossRef]
    [Google Scholar]
  7. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496
    [Google Scholar]
  8. Baumann P., Schubert R. H. W. 1983; Vibrionaceae . In Bergey's Manual of Systematic Bacteriology vol. 1 pp 516–550Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  9. Bertone S., Giacomini M., Ruggiero C., Piccarolo C., Calegari L. 1996; Automated systems for identification of heterotrophic marine bacteria on the basis of their fatty acid composition. Appl Environ Microbiol 62:2122–2132
    [Google Scholar]
  10. Farmer J. J. 1992; The family Vibrionaceae . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification and Applications pp 2938–2951Edited by Balows A. New York: Springer-Verlag;
    [Google Scholar]
  11. Galtier N., Gouy M., Gautier C. 1996; seaview and phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12:543–548
    [Google Scholar]
  12. Gomez-Gil B., Roque A., Turnbull J. F. 2000; The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquaculture 191:259–270 [CrossRef]
    [Google Scholar]
  13. Hjeltnes B., Roberts R. J. 1993; Vibriosis. In Bacterial Diseases of Fish pp 109–121Edited by Inglis V., Roberts R. J., Bromage N. R. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  14. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetic analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  15. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. In Current Methods for Classification and Identification of Microorganisms pp 1–67Edited by Colwell R. R., Grigorova R. London: Academic Press;
    [Google Scholar]
  16. Lightner D. V. 1993; Diseases of cultured penaeid shrimp. In CRC Handbook of Mariculture: Crustacean Aquaculture . , 2nd edn. vol 1 pp 393–486Edited by McVey J. P. Boca Raton, FL: CRC Press;
  17. Logan N. A., Lebbe L., Hoste B.7 other authors 2000; Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50:1741–1753
    [Google Scholar]
  18. Maidak B. L., Cole J. R., Parker C. T. Jr11 other authors 1999; A new version of the RDP (Ribosomal Database Project. Nucleic Acids Res 27:171–173 [CrossRef]
    [Google Scholar]
  19. McFall-Ngai M. J. 2002; Unseen forces: the influence of bacteria on animal development. Dev Biol 242:1–14 [CrossRef]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  21. Novoa B., Luque A., Castro D., Borrego J. J., Figueras A. 1998; Characterization and infectivity of four bacterial strains isolated from brown ring disease-affected clams. J Invertebr Pathol 71:34–41 [CrossRef]
    [Google Scholar]
  22. Osterhout G. J., Shull V. H., Dick J. D. 1991; Identification of clinical isolates of gram-negative nonfermentative bacteria by an automated cellular fatty acid identification system. J Clin Microbiol 29:1822–1830
    [Google Scholar]
  23. Pujalte M.-J., Ortigosa M., Urdaci M.-C., Garay E., Grimont P. A. D. 1993; Vibrio mytili sp. nov., from mussels. Int J Syst Bacteriol 43:358–362 [CrossRef]
    [Google Scholar]
  24. Raguénès G., Christen R., Guezennec J., Pignet P., Barbier G. 1997; Vibrio diabolicus sp. nov., a new polysaccharide-secreting organism isolated from a deep-sea hydrothermal vent polychaete annelid, Alvinella pompejana . Int J Syst Bacteriol 47:989–995 [CrossRef]
    [Google Scholar]
  25. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  27. Thompson F. L., Hoste B., Vandemeulebroecke K., Swings J. 2001; Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst Appl Microbiol 24:520–538 [CrossRef]
    [Google Scholar]
  28. Thompson F. L., Thompson C. C., Li Y., Gomez-Gil B., Vandenberghe J., Hoste B., Swings J. 2003; Vibrio kanaloae sp. nov, Vibrio pomeroyi sp. nov. and Vibrio chagasii sp. nov., from sea water and marine animals. Int J Syst Evol Microbiol 53:753–759 [CrossRef]
    [Google Scholar]
  29. Vandenberghe J., Li Y., Verdonck L., Li J., Sorgeloos P., Xu H. S., Swings J. 1998; Vibrios associated with Penaeus chinensis ( Crustacea : Decapoda ) larvae in Chinese shrimp hatcheries. Aquaculture 169:121–132 [CrossRef]
    [Google Scholar]
  30. Vandenberghe J., Thompson F. L., Gomez-Gil B., Swings J. 2003; Phenotypic diversity amongst Vibrio isolates from marine aquaculture systems. Aquaculture 219:9–20 [CrossRef]
    [Google Scholar]
  31. Willems A., Doignon-Bourcier F., Goris J., Coopman R., de Lajudie P., De Vos P., Gillis M. 2001; DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51:1315–1322
    [Google Scholar]
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