1887

Abstract

Two anaerobic, benzaldehyde-converting bacteria were isolated from an anaerobic upflow anaerobic sludge bed (UASB)-reactor treating potato starch waste water. Strain BOR-Y converted benzaldehyde to benzoate and benzylalcohol in approximately equimolar concentrations. Benzaldehyde conversion did not support growth. Strain BOR-Y was Gram-positive and rod-shaped, and its cells were slightly thickened in the middle. The strain was a mesophilic spore-former that grew between 15 and 40 °C, with optimum growth at 30–37 °C. The optimum pH for growth was pH 7·0. Strain BOR-Y grew on a wide range of carbohydrates and some other carbon sources including yeast extract, cysteine and serine. The G+C content of its DNA was 42 mol%. According to physiological characteristics and 16S rRNA gene sequence analysis, confirmed by DNA–DNA hybridization with its phylogenetic neighbours, strain BOR-Y belongs to a novel genus of cluster XII of the clostridia, namely ; the name is proposed for the type species (type strain BOR-Y=DSM 12858=ATCC BAA-502). Strain BR-10 reduced benzaldehyde to benzylalcohol. This conversion was coupled to growth. In a medium containing yeast extract, the presence of benzaldehyde resulted in the accumulation of more than twofold more cells. Strain BR-10 was a Gram-positive organism that was characterized by oval- or rod-shaped cells with oval ends, which occurred singly, in pairs or sometimes in chains. The strain was moderately thermophilic and grew between 20 and 60 °C, with optimum growth at 45 °C. The optimum pH for growth was between pH 7·0 and 7·5. Strain BR-10 grew on a wide range of carbon sources including carbohydrates, yeast extract, casein and some amino acids. The G+C content of its DNA was 32 mol%. As determined by 16S rRNA gene sequence analysis, strain BR-10 represents a novel species of cluster XIVa of the clostridia; the name is proposed for this novel species (type strain BR-10=DSM 12857=ATCC BAA-501).

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2003-11-01
2020-01-25
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vol. , part 6, pp. 1791–1799

Electron micrographs of BOR-YT (Fig. I) and BR-10T (Fig. II) are available when you click here. (PDF format)



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