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Abstract

A novel hydrocarbon-degrading, Gram-negative, obligately aerobic, non-motile, non-sporulating, rod-shaped bacterium, designated strain TBF2/20.2, was isolated from a biofilter clean-up facility set up on a hydrocarbon-contaminated site in Hungary. It was characterized by using a polyphasic approach to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate is affiliated with the genus in the family . It was found to be related most closely to Gsoil 060 (93.3 % 16S rRNA gene sequence similarity). Strain TBF2/20.2 grew at pH 6–9 (optimally at pH 6.5–7.0) and at 15–42 °C (optimally at 30–37 °C). The major fatty acids were iso-C (39.4 %), summed feature 3 (iso-C 2-OH and/or Cω7; 26.0 %), iso-C 3-OH (14.5 %) and C (4.5 %). The major menaquinone was MK-7 and the predominant polar lipid was phosphatidylethanolamine. The DNA G+C content of strain TBF2/20.2 was 41.2 mol%. Physiological and chemotaxonomic data further confirmed the distinctiveness of strain TBF2/20.2 from recognized members of the genus . Thus, strain TBF2/20.2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TBF2/20.2 ( = NCAIM B 02393  = CCM 7765).

Funding
This study was supported by the:
  • , Agruniver Holding Ltd , (Award KM_FOKT_06_ KMKINNOV)
  • , National Development Agency, Hungary , (Award KMOP 1.1.1-07/1)
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2011-12-01
2021-03-05
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