1887

Abstract

Comparative analysis of 16S rDNA sequences revealed a close phylogenetic relationship (99·6 % similarity) between Behrendt . 2002 and Evtushenko . 2001 . The two species were found to share genus-specific chemotaxonomic characteristics such as the occurrence of -ornithine and -2,4-diaminobutyric acid in the peptidoglycan and the profile of cellular fatty acids and 1,1-dimethoxy-alkanes. DNA–DNA relatedness of only 47·8 % and differences in phenotypic features such as the menaquinone profile and oxidase and Voges–Proskauer reactions confirmed the distinct species status of and . On the basis of the data from phylogenetic and phenotypic analyses, the reclassification of as comb. nov. is proposed. As a result of this reclassification, the two genera are coherent, in that the cell wall composition and 1,1-dimethoxy-alkane spectrum are significant genus-specific characteristics.

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2003-11-01
2021-03-07
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References

  1. Behrendt U., Ulrich A., Schumann P., Naumann D., Suzuki K. 2002; Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp. nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov. sp. nov. Int J Syst Evol Microbiol 52:1441–1454 [CrossRef]
    [Google Scholar]
  2. DSMZ 2001 Catalogue of Strains , 7th edn. Braunschweig: Deutsche Sammlung von Mikroorganismen und Zellkulturen;
    [Google Scholar]
  3. Escara J. F., Hutton J. R. 1980; Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19:1315–1327 [CrossRef]
    [Google Scholar]
  4. Evtushenko L. I., Dorofeeva L. V., Dobrovolskaya T. G., Streshinskaya G. M., Subbotin S. A., Tiedje J. M. 2001; Agreia bicolorata gen. nov., sp. nov. to accommodate actinobacteria isolated from narrow reed grass infected by the nematode Heteroanguina graminophila . Int J Syst Evol Microbiol 51:2073–2079 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1993 phylip (Phylogeny Interference Package), version 3.5c. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  7. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  8. Jahnke K. D. 1992; Basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73 [CrossRef]
    [Google Scholar]
  9. Männistö M. K., Schumann P., Rainey F. A., Kämpfer P., Tsitko I., Tiirola M. A., Salkinoja-Salonen M. S. 2000; Subtercola boreus gen. nov., sp. nov. and Subtercola frigoramans sp. nov., two new psychrophilic actinobacteria isolated from boreal groundwater. Int J Syst Evol Microbiol 501731–1739
    [Google Scholar]
  10. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  11. Sasaki J., Chijimatsu M., Suzuki K. 1998; Taxonomic significance of 2,4-diaminobutyric acid isomers in the cell wall peptidoglycan of actinomycetes and reclassification of Clavibacter toxicus as Rathayibacter toxicus comb. nov. Int J Syst Bacteriol 48:403–410 [CrossRef]
    [Google Scholar]
  12. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  13. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  14. Schumann P., Prauser H., Rainey F. A., Stackebrandt E., Hirsch P. 1997; Friedmanniella antarctica gen. nov., sp. nov. an ll-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol 47:278–283 [CrossRef]
    [Google Scholar]
  15. Stackebrandt E., Breymann S., Steiner U., Prauser H., Weiss N., Schumann P. 2002; Re-evaluation of the status of the genus Oerskovia , reclassification of Promicromonospora enterophila (Jáger et al . 1983) as Oerskovia enterophila comb. nov. and description of Oerskovia jenensis sp. nov. and Oerskovia paurometabola sp. nov. Int J Syst Evol Microbiol 52:1105–1111 [CrossRef]
    [Google Scholar]
  16. Takeuchi M., Hatano K. 1998; Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al . in a redefined genus Microbacterium . Int J Syst Bacteriol 48:739–747 [CrossRef]
    [Google Scholar]
  17. Uchida K., Kudo T., Suzuki K., Nakase T. 1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56 [CrossRef]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
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