1887

Abstract

A novel Gram-positive, anaerobic and thermophilic bacterium, strain MET79, was isolated from an oil well located in the Gulf of Mexico. Cells were straight rods, motile by a subpolar flagellum. Spores were formed in old cultures. Inner gas vacuoles swelled the cells when exposed to air. The optimum growth conditions were 55 °C, pH 7·5 and 1 % NaCl. Yeast extract was required for growth. Strain MET79 fermented several sugars, some organic acids and Casamino acids. Glucose was fermented into lactate, acetate, butyrate, H and CO. Strain MET79 reduced thiosulfate to hydrogen sulfide and nitrate to ammonium. The DNA G+C content was 30·9 mol%. The closest phylogenetic relative of strain MET79 was (88·7 % 16S rDNA sequence similarity). As strain MET79 (=DSM 15102=CIP 107615) was physiologically and phylogenetically different from its closest relatives, it is assigned as the type strain of a novel species of a new genus, gen. nov., sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02662-0
2003-09-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/5/ijs531509.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02662-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Balch, W. E., Fox, G. E., Magrum, R. J., Woese, C. R. & Wolfe, R. S. ( 1979; ). Methanogens: reevaluation of a unique biological group. Microbiol Rev 43, 260–296.
    [Google Scholar]
  3. Benson, D. A., Boguski, M. S., Lipman, D. J., Ostell, J., Ouellette, B. F. F., Rapp, B. A. & Wheeler, D. L. ( 1999; ). GenBank. Nucleic Acids Res 27, 12–17.[CrossRef]
    [Google Scholar]
  4. Bozzola, J. J. & Russell, L. D. ( 1991; ). Electron microscopy. In Principles and Techniques for Biologists, pp. 40–61. Boston: Jones & Bartlett.
  5. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. E. ( 1994; ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812–826.[CrossRef]
    [Google Scholar]
  6. Cord-Ruwisch, R. ( 1985; ). A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 4, 33–36.[CrossRef]
    [Google Scholar]
  7. Fardeau, M.-L., Ollivier, B., Patel, B. K. C., Magot, M., Thomas, P., Rimbault, A., Rocchiccioli, F. & Garcia, J.-L. ( 1997; ). Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. Int J Syst Bacteriol 47, 1013–1019.[CrossRef]
    [Google Scholar]
  8. Fardeau, M.-L., Magot, M., Patel, B. K. C., Thomas, P., Garcia, J.-L. & Ollivier, B. ( 2000; ). Thermoanaerobacter subterraneus sp. nov., a novel thermophile isolated from oilfield water. Int J Syst Evol Microbiol 50, 2141–2149.[CrossRef]
    [Google Scholar]
  9. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  10. Gevertz, D., Telang, A. J., Voordouw, G. & Jenneman, G. E. ( 2000; ). Isolation and characterization of strains CVO and FWKO B, two novel nitrate-reducing, sulfide-oxidizing bacteria isolated from oil field brine. Appl Environ Microbiol 66, 2491–2501.[CrossRef]
    [Google Scholar]
  11. Greene, A. C., Patel, B. K. C. & Sheehy, A. J. ( 1997; ). Deferribacter thermophilus gen. nov., sp. nov., a novel thermophilic manganese- and iron-reducing bacterium isolated from a petroleum reservoir. Int J Syst Bacteriol 47, 505–509.[CrossRef]
    [Google Scholar]
  12. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98 NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  13. Hungate, R. E. ( 1969; ). A roll-tube method for the cultivation of strict anaerobes. Methods Microbiol 3B, 117–132.
    [Google Scholar]
  14. Huu, N. B., Denner, E. B. M., Ha, D. T. C., Wanner, G. & Stan-Lotter, H. ( 1999; ). Marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a Vietnamese oil-producing well. Int J Syst Bacteriol 49, 367–375.[CrossRef]
    [Google Scholar]
  15. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 211–232. Edited by H. N. Munro. New York: Academic Press.
  16. Koussémon, M., Combet-Blanc, Y., Patel, B. K. C., Cayol, J.-L., Thomas, P., Garcia, J.-L. & Ollivier, B. ( 2001; ). Propionibacterium microaerophilum sp. nov., a microaerophilic bacterium isolated from olive mill wastewater. Int J Syst Evol Microbiol 51, 1373–1382.
    [Google Scholar]
  17. Magot, M., Ravot, G., Campaignolle, X., Ollivier, B., Patel, B. K. C., Fardeau, M.-L., Thomas, P., Crolet, J.-L. & Garcia, J.-L. ( 1997; ). Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a new anaerobic, slightly halophilic, thiosulfate-reducing bacterium from corroding offshore oil wells. Int J Syst Bacteriol 47, 818–824.[CrossRef]
    [Google Scholar]
  18. Magot, M., Ollivier, B. & Patel, B. K. C. ( 2000; ). Microbiology of petroleum reservoirs. Antonie van Leeuwenhoek 77, 103–116.[CrossRef]
    [Google Scholar]
  19. Maidak, B. L., Cole, J. R. & Lilburn, T. G. ( 2001; ). The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29, 173–174.[CrossRef]
    [Google Scholar]
  20. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  21. Myhr, S. & Torsvik, T. ( 2000; ). Denitrovibrio acetiphilus, a novel genus and species of dissimilatory nitrate-reducing bacterium isolated from an oil reservoir model column. Int J Syst Evol Microbiol 50, 1611–1619.[CrossRef]
    [Google Scholar]
  22. Ravot, G., Magot, M., Fardeau, M.-L., Patel, B. K. C., Prensier, G., Egan, A., Garcia, J.-L. & Ollivier, B. ( 1995a; ). Thermotoga elfii sp. nov., a novel thermophilic bacterium from an African oil-producing well. Int J Syst Bacteriol 45, 308–314.[CrossRef]
    [Google Scholar]
  23. Ravot, G., Ollivier, B., Magot, M., Patel, B. K. C., Crolet, J.-L., Fardeau, M.-L. & Garcia, J.-L. ( 1995b; ). Thiosulfate reduction, an important physiological feature shared by members of the order Thermotogales. Appl Environ Microbiol 61, 2053–2055.
    [Google Scholar]
  24. Ravot, G., Magot, M., Fardeau, M.-L., Patel, B. K. C., Thomas, P., Garcia, J.-L. & Ollivier, B. ( 1999; ). Fusibacter paucivorans gen. nov., sp. nov., an anaerobic, thiosulfate-reducing bacterium from an oil-producing well. Int J Syst Bacteriol 49, 1141–1147.[CrossRef]
    [Google Scholar]
  25. Rees, G. N., Patel, B. K. C., Grassia, G. S. & Sheehy, A. J. ( 1997; ). Anaerobaculum thermoterrenum gen. nov., sp. nov., a novel, thermophilic bacterium which ferments citrate. Int J Syst Bacteriol 47, 150–154.[CrossRef]
    [Google Scholar]
  26. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  27. Telang, A. J., Ebert, S., Foght, J. M., Westlake, D. W. S., Jenneman, G. E., Gevertz, D. & Voordouw, G. ( 1997; ). Effect of nitrate injection on the microbial community in an oil field as monitored by reverse sample genome probing. Appl Environ Microbiol 63, 1785–1793.
    [Google Scholar]
  28. Voordouw, G., Armstrong, S. M., Reimer, M. F., Fouts, B., Telang, A. J., Shen, Y. & Gevertz, D. ( 1996; ). Characterization of 16S rRNA genes from oil field microbial communities indicates the presence of a variety of sulfate-reducing, fermentative, and sulfide-oxidizing bacteria. Appl Environ Microbiol 62, 1623–1629.
    [Google Scholar]
  29. Wery, N., Moricet, J.-M., Cueff, V., Jean, J., Pignet, P., Lesongeur, F., Cambon-Bonavita, M.-A. & Barbier, G. ( 2001; ). Caloranaerobacter azorensis gen. nov., sp. nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 1789–1796.[CrossRef]
    [Google Scholar]
  30. Winker, S. & Woese, C. R. ( 1991; ). A definition of the domain Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA characteristics. Syst Appl Microbiol 13, 161–165.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02662-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02662-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error