1887

Abstract

On the basis of data from phenotypic and genotypic characterization and analysis of 16S rRNA gene sequences, two novel species belonging to the genus are described. Strains KMM 3584, a pale-yellowish, non-motile strain isolated from a starfish (), and KMM 3554, which is motile by means of a single subpolar flagellum and was isolated from sea grass (), are marine, Gram-negative, aerobic, rod-shaped organisms. Both strains have the ability to degrade gelatin, but not casein, chitin, agar, DNA, Tween 80 or starch. Strain KMM 3584 decomposed alginate and grew at NaCl concentrations of 1–8 % and temperatures of 12–37°C, whereas strain KMM 3554 grew in 1–12% NaCl and at temperatures of 10–30°C. The predominant fatty acid was 18:17, amounting to up to 80% of the total fatty acids. The other characteristic feature was the presence of 18:2 isomers. The DNA G+C contents of KMM 3584 and KMM 3554 were respectively 60·0 and 63·7 mol%. The level of DNA similarity between the two strains was 33%. DNA from KMM 3584 and KMM 3554 had hybridization values of 5–24% and 10–41%, respectively, with DNA from the type strains of , , and . It is proposed that strains KMM 3584 (=LMG 20554=ATCC BAA-321) and KMM 3554 (=LMG 20555=ATCC BAA-320) represent two novel species, sp. nov. and sp. nov., respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02654-0
2004-03-01
2020-10-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/2/ijs540475.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02654-0&mimeType=html&fmt=ahah

References

  1. Berry V., Gascuel O. 1996; Interpretation of bootstrap trees: threshold of clade selection and induced gain. Mol Biol Evol 13:999–1011 [CrossRef]
    [Google Scholar]
  2. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [CrossRef]
    [Google Scholar]
  3. Christensen H., Angen Ø., Mutters R., Olsen J. E., Bisgaard M. 2000; DNA–DNA hybridization determined in micro-wells using covalent attachment of DNA. Int J Syst Evol Microbiol 50:1095–1102 [CrossRef]
    [Google Scholar]
  4. Gascuel O. 1997; BIONJ: an improved version of the NJ algorithm based on a simple model of sequence data. Mol Biol Evol 14:685–695 [CrossRef]
    [Google Scholar]
  5. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66:24–26
    [Google Scholar]
  6. Ivanova E. P., Kiprianova E. A., Mikhailov V. V., Levanova F. G., Garagulya A. G., Gorshkova N. M., Yumoto N., Yoshikawa S. 1996; Characterization and identification of marine Alteromonas nigrifaciens strains and emendation of the description. Int J Syst Bacteriol 46:223–228 [CrossRef]
    [Google Scholar]
  7. Ivanova E. P., Kiprianova E. A., Mikhailov V. V. 8 other authors 1998; Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48:247–256 [CrossRef]
    [Google Scholar]
  8. Ivanova E. P., Romanenko L. A., Chun J. 7 other authors 2000; Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including description of two species, Idiomarina abyssalis sp.nov. and Idiomarina zobellii sp. nov. Int J Syst Evol Microbiol 50:901–907 [CrossRef]
    [Google Scholar]
  9. Kates M. 1986 Techniques in Lipidology New York: Elsevier;
    [Google Scholar]
  10. Kóvacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703
    [Google Scholar]
  11. Kurilenko V. V., Ivanova E. P., Mikhailov V. V. 2001; Zonal distribution of epiphytic microorganisms on the sea grass Zostera marina . Mikrobiologiya 70:427–428 (in Russian
    [Google Scholar]
  12. Labrenz M., Tindall B. J., Lawson P. A., Collins M. D., Schumann P., Hirsch P. 2000; Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., α -3- Proteobacteria from hypersaline, heliothermal and meromictic Antarctic Ekho Lake. Int J Syst Evol Microbiol 50:303–313 [CrossRef]
    [Google Scholar]
  13. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  14. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4:109–118
    [Google Scholar]
  15. Perrière G., Gouy M. 1996; WWW-query: an on-line retrieval system for biological sequence banks. Biochimie 78:364–369 [CrossRef]
    [Google Scholar]
  16. Pukall R., Buntefuß D., Frühling A., Rohde M., Kroppenstedt R. M., Burghardt J., Lebaron P, Bernard L, Stackebrandt E. 1999; Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α - Proteobacteria . Int J Syst Bacteriol 49:513–519 [CrossRef]
    [Google Scholar]
  17. Rüger H.-J., Krambeck H.-J. 1994; Evaluation of the BIOLOG substrate metabolism system for classification of marine bacteria. Syst Appl Microbiol 17:281–288 [CrossRef]
    [Google Scholar]
  18. Sawabe T., Sugimura I., Ohtsuka M., Nakano K., Tajima K., Ezura Y., Christen R. 1998a; Vibrio halioticoli sp. nov., a non-motile alginolytic marine bacterium isolated from the gut of the abalone Haliotis discus hannai . Int J Syst Bacteriol 48:573–580 [CrossRef]
    [Google Scholar]
  19. Sawabe T., Makino H., Tatsumi M., Nakano K., Tajima K., Iqbal M. M., Yumoto I., Ezura Y., Christen R. 1998b; Pseudoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of Laminaria japonica . Int J Syst Bacteriol 48:769–774 [CrossRef]
    [Google Scholar]
  20. Sawabe T., Tanaka R., Iqbal M. M., Tajima K., Ezura Y., Ivanova E. P., Christen R. 2000; Assignment of Alteromonas elyakovii KMM 162T and five strains isolated from spot-wounded fronds of Laminaria japonica to Pseudoalteromonas elyakovii comb. nov. and the extended description of the species. Int J Syst Evol Microbiol 50:265–271 [CrossRef]
    [Google Scholar]
  21. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Sorokin D. Y. 1995; Sulfitobacter pontiacus gen. nov., sp. nov. – a new heterotrophic bacterium from the Black Sea, specialized on sulfite oxidation. Mikrobiologiya 64:354–365 (English translation, 295–305)
    [Google Scholar]
  23. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium : new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
    [Google Scholar]
  24. Suzuki M. T., Rappe M. S., Haimberger Z. W., Winfield H., Adair N., Strobel J., Giovannoni S. J. 1997; Bacterial diversity among small-subunit rRNA gene clones and cellular isolates from the same seawater sample. Appl Environ Microbiol 63:983–989
    [Google Scholar]
  25. Svetashev V. I., Vaskovsky V. E. 1972; A simplified technique for thin-layer microchromatography of lipids. J Chromatogr 67:376–378 [CrossRef]
    [Google Scholar]
  26. Svetashev V. I., Vysotskii M. V., Ivanova E. P., Mikhailov V. V. 1995; Cellular fatty acids of Alteromonas species. Syst Appl Microbiol 18:37–43 [CrossRef]
    [Google Scholar]
  27. Vaskovsky V. E., Kostetsky E. Y., Vasendin I. M. 1975; A universal reagent for phospholipid analysis. J Chromatogr 114:129–141 [CrossRef]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02654-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02654-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF

Supplementary material 4

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error