gen. nov., sp. nov., from human faeces Free

Abstract

Phenotypic and phylogenetic studies were performed on two isolates of an unidentified Gram-positive, anaerobic, non-spore-forming, rod-shaped bacterium that was isolated from human faeces. The organisms were catalase-negative, produced acetic and butyric acids as end products of metabolism and possessed a DNA G+C content of approximately 54 mol%. Comparative 16S rRNA gene sequencing demonstrated that the two isolates were related closely to each other and formed a hitherto unknown sublineage within the rRNA cluster of organisms. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium should be classified in a novel genus as gen. nov., sp. nov. The type strain of is WAL 14565=CCUG 45055=CIP 107754.

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2004-03-01
2024-03-28
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References

  1. Barcenilla A., Pryde S. E., Martin J. C., Duncan S. H., Stewart C. S., Henderson C., Flint H. J. 2000; Phylogenetic relationships of butyrate-producing bacteria from the human gut. Appl Environ Microbiol 66:1654–1661 [CrossRef]
    [Google Scholar]
  2. Bryant M. P. 1986; Genus Ruminococcus . . In Bergey's Manual of Systematic Bacteriology vol 2 pp  1093–1097 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Cato E. P., Salmon C. W., Moore W. E. C. 1974; Fusobacterium prausnitzii (Hauduroy et al.) Moore and Holdeman: emended description and designation of neotype strain. Int J Syst Bacteriol 24:225–229 [CrossRef]
    [Google Scholar]
  4. Cato E. P., George W. L., Finegold S. M. 1986; Genus Clostridium Prazmowski 1880, 23AL . In Bergey's Manual of Systematic Bacteriology vol. 2 pp  1141–1200 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  5. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium : proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826 [CrossRef]
    [Google Scholar]
  6. Ducluzeau R. 1988; Role of experimental microbial ecology in gastroenterology. In Microbial Ecology and Intestinal Infections pp  7–26 Edited by Bergogne-Berezin E. New York: Academic Press;
    [Google Scholar]
  7. Duncan S. H., Hold G. L., Harmsen H. J. M., Stewart C. S., Flint H. J. 2002; Growth requirements and fermentation products of Fusobacterium prausnitzii , and a proposal to reclassify it as Faecalibacterium prausnitzii gen. nov., comb. nov.. Int J Syst Evol Microbiol 52:2141–2146 [CrossRef]
    [Google Scholar]
  8. Gibson G. R., Roberfroid M. B. 1995; Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125:1401–1412
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. (editors) 1977 Anaerobe Laboratory Manual , 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  10. Jousimies-Somer H. R., Summanen P., Citron D. M., Baron E. J., Wexler H. M., Finegold S. M. 2002 Wadsworth Anaerobic Bacteriology Manual , 6th edn. Belmont, CA: Star Publishing;
    [Google Scholar]
  11. Lipman D. J., Pearson W. R. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441 [CrossRef]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Moore W. E. C., Holdeman Moore L. V. 1986; Genus Eubacterium Prévot 1938. In Bergey's Manual of Systematic Bacteriology vol. 2 pp  1353–1373 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  14. Moore W. E. C., Johnson J. L., Holdeman L. V. 1976; Emendation of Bacteroidaceae and Butyrivibrio and descriptions of Desulfomonas gen. nov. and ten new species in the genera Desulfomonas , Butyrivibrio , Eubacterium , Clostridium , and Ruminococcus . Int J Syst Bacteriol 26:238–252 [CrossRef]
    [Google Scholar]
  15. Nicholas K. B., Nicholas H. B. Jr, Deerfield D. W. II 1997; GeneDoc: analysis and visualization of genetic variation. EMBNEW News 4:14
    [Google Scholar]
  16. Page R. D. M. 1996; treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  17. Rasmussen S. W. 1995 DNATools, a software package for DNA sequence analysis Carlsberg Laboratory; Copenhagen:
    [Google Scholar]
  18. Steer T., Collins M. D., Gibson G. R., Hippe H., Lawson P. A. 2001; Clostridium hathewayi sp. nov., from human faeces. Syst Appl Microbiol 24:353–357 [CrossRef]
    [Google Scholar]
  19. Suau A., Bonnet R., Sutren M., Godon J.-J., Gibson G. R., Collins M. D., Doré J. 1999; Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 65:4799–4807
    [Google Scholar]
  20. Taras D., Simmering R., Collins M. D., Lawson P. A., Blaut M. 2002; Reclassification of Eubacterium formicigenerans Holdeman and Moore 1974 as Dorea formicigenerans gen. nov., comb. nov., and description of Dorea longicatena sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 52:423–428
    [Google Scholar]
  21. Wilson K. H., Blitchington R. B. 1996; Human colonic biota studied by ribosomal DNA sequence analysis. Appl Environ Microbiol 62:2273–2278
    [Google Scholar]
  22. Yanling H., Youfang D., Yanquan L. 1991; Two cellulolytic Clostridium species: Clostridium cellulosi sp. nov. and Clostridium cellulofermentans sp. nov. Int J Syst Bacteriol 41:306–309 [CrossRef]
    [Google Scholar]
  23. Zellner G., Stackebrandt E., Nagel D., Messner P., Weiss N., Winter J. 1996; Anaerofilum pentosovorans gen. nov., sp. nov., and Anaerofilum agile sp. nov., two new, strictly anaerobic, mesophilic, acidogenic bacteria from anaerobic bioreactors. Int J Syst Bacteriol 46:871–875 [CrossRef]
    [Google Scholar]
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