Four coagulase-negative, novobiocin-resistant cocci, designated CW1T, PM34, MM3 and RW78, were isolated from the respiratory tract of goats kept in the Himalayan region. The four isolates were assigned to a single species on the basis of almost identical biochemical and physiological traits, protein profiles obtained after SDS-PAGE and identical genomic fingerprints generated after enterobacterial repetitive intergenic consensus (ERIC)-PCR. Strain CW1T showed highest 16S rDNA sequence similarities to Staphylococcus cohnii subsp. urealyticus ATCC 49330T, Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305T, S. cohnii subsp. cohnii ATCC 29974T, Staphylococcus arlettae ATCC 43957T, Staphylococcus gallinarum ATCC 35539T, Staphylococcus succinus ATCC 700337T and Staphylococcus xylosus ATCC 29971T (99·0, 98·8, 98·8, 98·4, 98·2, 98·1 and 98·1 %, respectively), indicating its classification within the genus Staphylococcus. The polar lipid composition, fatty acid profiles, quinone systems and diagnostic cell-wall diamino acid were in agreement with the characteristics of the genus Staphylococcus. DNA–DNA hybridization with closely related Staphylococcus species suggested that strain CW1T represents an as-yet unrecognized species. Based on these results, a novel species of the genus Staphylococcus is described, Staphylococcus nepalensis sp. nov. The type strain is CW1T (=DSM 15150T=CCM 7045T) and the most dissimilar strain is PM34 (=DSM 15151=CCM 7046).
Bedidi-MadaniN.,
GreenlandT.,
RichardY.1998; Exoprotein and slime production by coagulase-negative staphylococci isolated from goats' milk. Vet Microbiol 59:139–145[CrossRef]
BehmeR. J.,
ShuttleworthR.,
McNabbA.,
ColbyW. D.1996; Identification of staphylococci with a self-educating system using fatty acid analysis and biochemical tests. J Clin Microbiol 34:3075–3084
BrosiusJ.,
PalmerM. L.,
KennedyP. J.,
NollerH. F.1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805[CrossRef]
FerrerO.,
RealF.,
MolinaJ. M.,
AcostaB.,
MunozM. C.,
LeonL.1997; IgG concentration in mammary secretions of goats throughout lactation in healthy and coagulase-negative staphylococci infected udders. Comp Immunol Microbiol Infect Dis 20:253–260[CrossRef]
FreneyJ.,
KloosW. E.,
HajekV.,
WebsterJ. A.,
BesM.,
BrunY.,
Vernozy-RozandC.1999; Recommended minimal standards for description of new staphylococcal species. Subcommittee on the taxonomy of staphylococci and streptococci of the International Committee on Systematic Bacteriology. Int J Syst Bacteriol 49:489–502[CrossRef]
HájekV., LudwigW.,
SchleiferK. H.,
SpringerN.,
ZitzelsbergerW.,
KroppenstedtR. M.,
KocurM.1992; Staphylococcus muscae , a new species isolated from flies. Int J Syst Bacteriol 42:97–101[CrossRef]
HoltJ. G.,
KriegN. R.,
SneathP. H. A.,
StaleyJ. T.,
WilliamsS. T.editors1994Bergey's Manual of Determinative Bacteriology , 9th edn. Baltimore: Williams & Wilkins;
KloosW. E.,
SchleiferK. H.1986; Genus IV. Staphylococcus Rosenbach 1884, 18AL
. In Bergey's Manual of Systematic Bacteriologyvol. 2 pp 1013–1035Edited bySneathP. H. A.,
MairN. S.,
SharpeM. E.,
HoltJ. G.
Baltimore: Williams & Wilkins;
KloosW. E.,
SchleiferK. H.,
GötzF.1992; The genus Staphylococcus . In The Prokaryotes , 2nd edn. pp 1369–1420Edited byBalowsA.,
TrüperH. G., DworkinM.,
HarderW.,
SchleiferK. H.
New York: Springer;
KotilainenP.,
HuovinenP.,
EerolaE.1991; Application of gas-liquid chromatographic analysis of cellular fatty acids for species identification and typing of coagulase-negative staphylococci. J Clin Microbiol 29:315–322
MahantaP. N.,
ThungchamoK.,
DuttaG. N.,
DevrieseL. A.1997; Identification and characterization of staphylococci isolated from cutaneous lesions of goats. Zentbl Veterinarmed B 44:309–311
NahaieM. R.,
GoodfellowM.,
MinnikinD. E.,
HájekV.1984; Polar lipid and isoprenoid quinone composition in the classification of Staphylococcus . J Gen Microbiol 130:2427–2437
RosengartenR.,
BehrensA.,
StetefeldA.,
HellerM.,
AhrensM.,
SachseK.,
YogevD.,
KirchhoffH.1994; Antigen heterogeneity among isolates of Mycoplasma bovis is generated by high-frequency variation of diverse membrane surface proteins. Infect Immun 62:5066–5074
TakahashiT.,
SatohI.,
KikuchiN.1999; Phylogenetic relationships of 38 taxa of the genus Staphylococcus based on 16S rRNA gene sequence analysis. Int J Syst Bacteriol 49:725–728[CrossRef]
VentosaA.,
MarquezM. C.,
KocurM.,
TindallB. J.1993; Comparative study of “ Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus . Int J Syst Bacteriol 43:245–248[CrossRef]
WieserM.,
SchumannP.,
MartinK.,
AltenburgerP.,
BurghardtJ.,
LubitzW.,
BusseH.-J.1999; Agrococcus citreus sp. nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria. Int J Syst Bacteriol 49:1165–1170[CrossRef]
ZiemkeF.,
HöfleM. G., LalucatJ.,
Rosselló-MoraR.1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186[CrossRef]