1887

Abstract

Three brownish-yellow bacterial strains were isolated from the western Sargasso Sea by high-throughput culturing methods and characterized by polyphasic approaches. All isolates were Gram-negative, strictly aerobic, chemoheterotrophic, non-motile short rods that contained carotenoid pigments. Phylogenetic analyses based on 16S rRNA gene sequences, DNA–DNA hybridization and DNA G+C content, along with phenotypic characteristics, revealed that they belonged to the same species. The strains utilized a wide range of substrates, including pentoses, hexoses, oligosaccharides, sugar alcohols, organic acids and amino acids, as sole carbon sources. The DNA G+C content of the isolates ranged from 57·6 to 59·9 mol%. The predominant cellular fatty acid constituent was C 7, whilst C, C and C 8 cyclo were also abundant. The organism related most closely to these strains, as determined by 16S rDNA sequence comparison, was the recently described species (93·3–93·8 % similarity). Phylogenetic analyses indicated that the strains formed a distinct and deep evolutionary lineage of descent, together with , within the order ‘’ of the -. This lineage could not be associated with any of the ten known families in the order ‘’. From polyphasic evidence, it is proposed that the strains be placed into a novel genus and species, gen. nov., sp. nov. (type strain, HTCC2506=ATCC BAA-666=KCTC 12091=DSM 15513).

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2003-11-01
2020-08-05
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vol. , part 6, pp. 1853 – 1859

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