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Volume 61, Issue 9

sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant Free

Abstract

A Gram-negative-staining, motile, rod-shaped, aerobic bacterial strain, designated MJ02, was isolated from sludge of a leachate treatment plant in Daejeon (South Korea) and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ02 belonged to the family , class , and was most closely related to MS4 (97.6 % sequence similarity) and DC-196 (97.5 %). The G+C content of the genomic DNA of strain MJ02 was 64.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone and a fatty acid profile with Cω7 (45.8 %) and C (21.8 %) as the major components supported the affiliation of strain MJ02 to the genus . However, strain MJ02 exhibited relatively low levels of DNA–DNA relatedness with respect to DSM 21319 (17±7 %) and KACC 14483 (12±6 %), showing clearly that the isolate constituted a new genospecies. Strain MJ02 could be clearly differentiated from its phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ02 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MJ02 ( = KCTC 22450 = JCM 16236).

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Funding
This study was supported by the:
  • Ministry of Environment of the Republic of Korea (Award 071-081-133)
© 2011 IUMS
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2011-09-01
2024-04-19
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Shinella daejeonensis sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant
Int J Syst Evol Microbiol 61, 2123 (2011); https://doi.org/10.1099/ijs.0.026435-0
/content/journal/ijsem/10.1099/ijs.0.026435-0
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