1887

Abstract

The taxonomic position, growth characteristics and antibiotic resistance properties of a slightly yellow-pigmented bacterial strain, designated R26, isolated from the midgut of the mosquito , were studied. The isolate produced rod-shaped cells, which stained Gram-negative. The bacterium had two growth optima at 30–31 °C and 37 °C. Strain R26 demonstrated natural antibiotic resistance to ampicillin, chloramphenicol, kanamycin, streptomycin and tetracycline. 16S rRNA gene sequence analysis revealed that the isolate showed 98.6 % sequence similarity to that of ATCC 13253 and 98.2 % similarity to that of GTC 862. The major fatty acids of strain R26 were iso-C, iso-C 3-OH and summed feature 4 (iso-C 2-OH and/or Cω7/). Strain R26 contained only menaquinone MK-6 and showed a complex polar lipid profile consisting of diphosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and unknown polar lipids and glycolipids. DNA–DNA hybridization experiments with CCUG 214 ( = ATCC 13253) and KCTC 12492 ( = GTC 862) gave relatedness values of 34.5 % (reciprocal 41.5 %) and 35.0 % (reciprocal 25.7 %), respectively. DNA–DNA hybridization results and some differentiating biochemical properties indicate that strain R26 represents a novel species, for which the name sp. nov. is proposed. The type strain is R26 ( = CCUG 60038  = CCM 7804).

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2011-11-01
2020-01-21
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