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Abstract

We have undertaken a polyphasic taxonomic study of two halophilic, Gram-negative bacterial strains, N12 and B-100, that produce sulphated exopolysaccharides with biological activity. They were isolated from two different saline soil samples. Both strains grow at NaCl concentrations within the range 3–15 % (w/v) [optimum 5–10 % (w/v)], at 15–37 °C (optimum 20–32 °C) and at pH 6–8 (optimum pH 7–8). Their 16S rRNA gene sequences indicate that they belong to the genus in the class . Their closest relative is , to which our strains show maximum 16S rRNA gene sequence similarity values of 98.7 % (N12) and 98.3 % (B-100). Their DNA G+C contents are 61.9 and 63.8 mol%, respectively. The results of DNA–DNA hybridizations showed 43.9 % relatedness between strain N12 and CECT 7281, 30.5 % between N12 and CECT 5797, 39.2 % between N12 and CECT 7341, 46.3 % between N12 and CECT 5298, 52.9 % between N12 and LMG 23976, 51.3 % between N12 and JCM 12237 and 100 % between strains N12 and B-100. The major fatty acids of strain N12 are C 3-OH (5.42 %), C iso 2-OH/Cω7 (17.37 %), C (21.62 %) and Cω7 (49.19 %). The proposed name for the novel species is sp. nov Strain N12 ( = CECT 7744  = LMG 25812) is the type strain.

Funding
This study was supported by the:
  • Spanish Ministerio de Educación y Ciencia (Award CGL2008-02399/BOS and CGL 2005-05947)
  • Consejería de Educación, Ciencia y Empresa, Junta de Andalucía (Award P06-CVI-01850)
  • Plan Andaluz de Investigación, Spain
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2011-10-01
2024-10-12
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