1887

Abstract

Two novel Gram-negative, oxidase- and catalase-positive, rod-shaped bacterial strains, designated YCSA28 and YCSA39, were isolated from sediment of Daqiao saltern, Jimo, Qingdao, on the east coast of China. The two strains grew optimally at 28–30 °C, at pH 7.5 and in the presence of 7–8 % (w/v) NaCl. They were assigned to the genus , class , based on 16S rRNA gene sequence analysis. The major cellular fatty acids of the two strains were Cω7 (42.9 %), C (23.1 %) and Cω7/ω6 (18.0 %), and Q-9 was the major ubiquinone. The G+C content of the DNA of strains YCSA28 and YCSA39 was 63.7 and 63.9 mol%, respectively. The predominant respiratory lipoquinone, cellular fatty acid profiles and DNA G+C content of strains YCSA28 and YCSA39 were consistent with those of recognized species of the genus . Levels of DNA–DNA relatedness between strains YCSA28 and YCSA39, between YCSA28 and Al12, and between YCSA39 and Al12 were 95, 45 and 50 %, respectively. Together, these data indicated that strains YCSA28 and YCSA39 represent a single novel species of the genus , for which the name sp. nov. is proposed. The type strain is YCSA28 ( = CGMCC 1.9150  = NCCB 100305  = MCCC 1B00920).

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2011-07-01
2019-10-20
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References

  1. Akagawa M. , Yamasato K. . ( 1989; ). Synonymy of Alcaligenes aquamarinus, Alcaligenes faecalis subsp. homari, and Deleya aesta: Deleya aquamarina comb. nov. as the type species of the genus Deleya . . Int J Syst Bacteriol 39:, 462–466. [CrossRef]
    [Google Scholar]
  2. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed].[CrossRef]
    [Google Scholar]
  3. Arahal D. R. , Vreeland R. H. , Litchfield C. D. , Mormile M. R. , Tindall B. J. , Oren A. , Béjar V. , Quesada E. , Ventosa A. . ( 2007; ). Recommended minimal standards for describing new taxa of the family Halomonadaceae . . Int J Syst Evol Microbiol 57:, 2436–2446. [CrossRef].[PubMed].
    [Google Scholar]
  4. Benson D. A. , Karsch-Mizrachi I. , Lipman D. J. , Ostell J. , Sayers E. W. . ( 2009; ). Genbank. . Nucleic Acids Res 37: Suppl 1 D26–D31. [CrossRef].[PubMed].
    [Google Scholar]
  5. Berendes F. , Gottschalk G. , Heine-Dobbernack E. , Moore E. R. B. , Tindall B. J. . ( 1996; ). Halomonas desiderata sp. nov., a new alkaliphilic, halotolerant and denitrifying bacterium isolated from a municipal sewage works. . Syst Appl Microbiol 19:, 158–167.[CrossRef]
    [Google Scholar]
  6. Buck J. D. . ( 1982; ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44:, 992–993.[PubMed].
    [Google Scholar]
  7. Dobson S. J. , Franzmann P. D. . ( 1996; ). Unification of the genera Deleya (Bauman et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae . . Int J Syst Bacteriol 46:, 550–558. [CrossRef]
    [Google Scholar]
  8. Dong X.-Z. , Cai M.-Y. . (editors) ( 2001; ). Determinative Manual for Routine Bacteriology. Beijing:: Scientific Press;.
    [Google Scholar]
  9. González-Domenech C. M. , Béjar V. , Martínez-Checa F. , Quesada E. . ( 2008; ). Halomonas nitroreducens sp. nov., a novel nitrate- and nitrite-reducing species. . Int J Syst Evol Microbiol 58:, 872–876. [CrossRef].[PubMed].
    [Google Scholar]
  10. González-Domenech C. M. , Martínez-Checa F. , Quesada E. , Béjar V. . ( 2009; ). Halomonas fontilapidosi sp. nov., a moderately halophilic, denitrifying bacterium. . Int J Syst Evol Microbiol 59:, 1290–1296. [CrossRef].[PubMed].
    [Google Scholar]
  11. Hiraishi A. . ( 1992; ). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. . Lett Appl Microbiol 15:, 210–213. [CrossRef].[PubMed].
    [Google Scholar]
  12. Kimura M. . ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;.[CrossRef]
    [Google Scholar]
  13. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  14. Lai Q. L. , Shao Z. Z. . ( 2008; ). Pseudomonas xiamenensis sp. nov., a denitrifying bacterium isolated from activated sludge. . Int J Syst Evol Microbiol 58:, 1911–1915. [CrossRef].[PubMed].
    [Google Scholar]
  15. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . . New York:: Wiley;.
    [Google Scholar]
  16. Li H. B. , Zhang L. P. , Chen S. F. . ( 2008; ). Halomonas korlensis sp. nov., a moderately halophilic, denitrifying bacterium isolated from saline and alkaline soil. . Int J Syst Evol Microbiol 58:, 2582–2588. [CrossRef].[PubMed].
    [Google Scholar]
  17. Liu C. , Shao Z. Z. . ( 2005; ). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. . Int J Syst Evol Microbiol 55:, 1181–1186. [CrossRef].[PubMed].
    [Google Scholar]
  18. Martínez-Cánovas M. J. , Quesada E. , Llamas I. , Béjar V. . ( 2004; ). Halomonas ventosae sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium. . Int J Syst Evol Microbiol 54:, 733–737. [CrossRef].[PubMed].
    [Google Scholar]
  19. Mata J. A. , Martínez-Cánovas J. , Quesada E. , Béjar V. . ( 2002; ). A detailed phenotypic characterisation of the type strains of Halomonas species. . Syst Appl Microbiol 25:, 360–375. [CrossRef].[PubMed].
    [Google Scholar]
  20. Mesbah M. , Whitman W. B. . ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479:, 297–306. [CrossRef].[PubMed].
    [Google Scholar]
  21. Moraine R. A. , Rogovin P. . ( 1966; ). Kinetics of polysaccharide B-1459 fermentation. . Biotechnol Bioeng 8:, 511–524. [CrossRef]
    [Google Scholar]
  22. Ostle A. G. , Holt J. G. . ( 1982; ). Nile blue A as a fluorescent stain for poly-β-hydroxybutyrate. . Appl Environ Microbiol 44:, 238–241.[PubMed].
    [Google Scholar]
  23. Qu L. Y. , Lai Q. L. , Zhu F. L. , Hong X.G. , Shao Z. Z. , Sun X. Q. . ( 2011; ). Cohaesibacter marisflavi sp. nov., a marine bacterium isolated from sediment of a seashore pond for sea cucumber culture. . Int J Syst Evol Microbiol 61:, 762–-766. [CrossRef]
    [Google Scholar]
  24. Rodríguez-Valera F. , Ruiz-Berraquero F. , Ramos-Cormenzana A. . ( 1981; ). Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentrations. . Microb Ecol 7:, 235–243. [CrossRef]
    [Google Scholar]
  25. Romanenko L. A. , Schumann P. , Rohde M. , Mikhailov V. V. , Stackebrandt E. . ( 2002; ). Halomonas halocynthiae sp. nov., isolated from the marine ascidian Halocynthia aurantium . . Int J Syst Evol Microbiol 52:, 1767–1772. [CrossRef]
    [Google Scholar]
  26. Rzhetsky A. , Nei M. . ( 1992; ). A simple method for estimating and testing minimum-evolution trees. . Mol Biol Evol 9:, 945–967.
    [Google Scholar]
  27. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed].
    [Google Scholar]
  28. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 . Newark, DE:: MIDI Inc;.
    [Google Scholar]
  29. Stackebrandt E. , Ebers J. . ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33:, 152–155.
    [Google Scholar]
  30. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef].[PubMed].
    [Google Scholar]
  31. Vreeland R. H. , Litchfield C. D. , Martin E. L. , Elliot E. . ( 1980; ). Halomonas elongata, a new genus and species of extremely salt tolerant bacteria. . Int J Syst Bacteriol 30:, 485–495. [CrossRef]
    [Google Scholar]
  32. Wang Y. N. , Cai H. , Chi C. Q. , Lu A. H. , Lin X. G. , Jiang Z. F. , Wu X. L. . ( 2007; ). Halomonas shengliensis sp. nov., a moderately halophilic, denitrifying, crude-oil-utilizing bacterium. . Int J Syst Evol Microbiol 57:, 1222–1226. [CrossRef].[PubMed].
    [Google Scholar]
  33. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  34. Yoon J. H. , Lee K. C. , Kho Y. H. , Kang K. H. , Kim C. J. , Park Y. H. . ( 2002; ). Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 52:, 123–130.[PubMed].[CrossRef]
    [Google Scholar]
  35. Zhou X. H. , Wang Y. , Wu M. . ( 2007; ). Isolation and exopolysaccharide screening of halophiles from Zhoushan Islands. . J Zhejiang Uni 34:, 335–339.
    [Google Scholar]
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Phylogenetic tree based on 16S rRNA gene sequences according to the minimum-evolution method (Rzhetsky & Nei, 1992), showing the positions of strains YCSA28 and YCSA39, and the type strains of related taxa within the family . Bootstrap values (expressed as percentages of 1000 replications) of >50 % are shown at branch points. Bar, 0.005 nt substitution rate ( ) units.

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. Transmission electron micrograph of a negatively stained cell of strain YCSA28 . Bar, 200 nm.

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