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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 53, Issue 5

sp. nov., a moderately halophilic actinomycete isolated from soil in China Free

Abstract

A novel, moderately halophilic actinomycete, strain YIM 90007, was isolated from a soil sample collected from the Xinjiang Province, China, and characterized. The optimum growth temperature of the strain was between 35 and 37 °C and growth occurred optimally in 10 % (w/v) NaCl. The cell wall of strain YIM 90007 contained -diaminopimelic acid. Whole-cell sugars were galactose, arabinose and ribose. The principal menaquinone was MK-9(H), while MK-9(H) was found in smaller amounts. The phospholipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and hydroxyphosphatidylethanolamine. The predominant cellular fatty acids were of the iso- and anteiso-branched and unbranched types; significant amounts of 2-hydroxy fatty acids were also found but 10-methyl-branched fatty acids were missing. The DNA G+C content of strain YIM 900007 was 71 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed strain YIM 90007 to be closely related to (98·7 % similarity). DNA–DNA hybridization revealed a relatedness of 53·8 % between strain YIM 90007 and DSM 44411. Based on physiological and biochemical characteristics, phylogenetic analysis (based on 16S rRNA gene sequences) and DNA–DNA relatedness, it is concluded that strain YIM 90007 represents a novel species of the genus , for which the name (type strain YIM 90007=CCTCC AA001018=CCRC 16315=DSM 44619) is proposed.

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2003-09-01
2025-04-23
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Saccharomonospora paurometabolica sp. nov., a moderately halophilic actinomycete isolated from soil in China
Int J Syst Evol Microbiol 53, 1591 (2003); https://doi.org/10.1099/ijs.0.02633-0
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