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Abstract

A Gram-negative, facultatively anaerobic, rod-shaped, catalase- and oxidase-positive bacterium, motile by means of a single polar flagellum and designated strain KJW27, was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was β-haemolytic and grew with 0–10 % (w/v) NaCl, at 10–45 °C and at pH 6.5–10, with optimum growth with 2 % (w/v) NaCl, at 37 °C and at pH 7.5. The major fatty acids were iso-C (22.2 %), Cω8 (21 %), summed feature 3 (comprising Cω7 and/or Cω6; 10.2 %), C (7.1 %), iso-C (5.6 %) and C (4.4 %). The DNA G+C content was 51.2 mol%. Phylogenetic analysis based on 16S rRNA and gene sequences showed that strain KJW27 forms a lineage within the genus and is closely related to ATCC 51192 (98.8 %), DW01 (98.8 %) and JC5 (98.2 %). Sequence identity with other members of this genus ranges from 92.2 to 96.4 %. The DNA–DNA relatedness of strain KJW27 with ATCC 51192, DW01 and JC5 was 52, 44 and 33 %, respectively. The phenotypic, genotypic and DNA–DNA relatedness data indicate that strain KJW27 should be distinguished from ATCC 51192, DW01 and JC5. On the basis of the data presented in this study, strain KJW27 represents a novel species, for which the name sp. nov. is proposed. The type strain is KJW27 ( = KCTC 23171  = BCC 41031  = NCIM 5388).

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2011-09-01
2019-10-15
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Supplements

vol. , part 9, pp. 2058 - 2064

Supplementary material [ PDF] (98 KB) containing:

Results from the GN VITEK-2 compact system v. 03.01 (bioMérieux)

Results from the GN2 Micro Plate (Biolog)

Activity of different antimicrobials

Cellular fatty acid compositions (%) of strain KJW27 and the type strains of phylogenetically related species of the genus

Neighbour-joining tree based on partial gene sequences showing the phylogenetic position of strain KJW27 and members of the genus

Maximum-parsimony tree based on nearly complete 16S rRNA gene sequences showing the relationships between strain KJW27 and members of the genus



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