1887

Abstract

The taxonomic status of seven glucose-non-acidifying, non-proteolytic strains characterized by forming small colonies on agar media was studied. With one exception, all strains were from human specimens. They could be distinguished from all described (genomic) species by their ability to grow on ethanol and acetate as sole sources of carbon but not on 22 other substrates tested including -lactate or -4-aminobutyrate. DNA–DNA hybridization studies, 16S rRNA gene sequence analysis, amplified rDNA restriction analysis and DNA polymorphism analysis by AFLP showed that these strains represent a hitherto unknown species of the genus , for which the name (type strain LMG 21765=LUH 4616=NIPH 384=CCM 7030) is proposed.

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2003-09-01
2019-10-22
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References

  1. Bernards, A. T., de Beaufort, A. J., Dijkshoorn, L. & van Boven, C. P. A. ( 1997; ). Outbreak of septicaemia in neonates caused by Acinetobacter junii investigated by amplified ribosomal DNA restriction analysis (ARDRA) and four typing methods. J Hosp Infect 35, 129–140.
    [Google Scholar]
  2. Bouvet, P. J. M. & Grimont, P. A. D. ( 1986; ). Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. Int J Syst Bacteriol 36, 228–240.[CrossRef]
    [Google Scholar]
  3. Bouvet, P. J. M. & Grimont, P. A. D. ( 1987; ). Identification and biotyping of clinical isolates of Acinetobacter. Ann Inst Pasteur Microbiol 138, 569–578.[CrossRef]
    [Google Scholar]
  4. Bouvet, P. J. M. & Jeanjean, S. ( 1989; ). Delineation of new proteolytic genomic species in the genus Acinetobacter. Res Microbiol 140, 291–299.[CrossRef]
    [Google Scholar]
  5. Carr, E. L., Kämpfer, P., Patel, B. K. C., Gürtler, V. & Seviour, R. J. ( 2003; ). Seven novel species of Acinetobacter isolated from activated sludge. Int J Syst Evol Microbiol 53, 953–963.[CrossRef]
    [Google Scholar]
  6. Cruze, J. A., Singer, J. T. & Finnerty, W. R. ( 1979; ). Conditions for quantitative transformation in Acinetobacter calcoaceticus. Curr Microbiol 3, 129–132.[CrossRef]
    [Google Scholar]
  7. Dijkshoorn, L., van Harsselaar, B., Tjernberg, I., Bouvet, P. J. M. & Vaneechoutte, M. ( 1998; ). Evaluation of amplified ribosomal DNA restriction analysis for identification of Acinetobacter genomic species. Syst Appl Microbiol 21, 33–39.[CrossRef]
    [Google Scholar]
  8. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  9. Gerner-Smidt, P. & Tjernberg, I. ( 1993; ). Acinetobacter in Denmark: II. Molecular studies of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. APMIS 101, 826–832.[CrossRef]
    [Google Scholar]
  10. Gerner-Smidt, P., Tjernberg, I. & Ursing, J. ( 1991; ). Reliability of phenotypic tests for identification of Acinetobacter species. J Clin Microbiol 29, 277–282.
    [Google Scholar]
  11. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K. ( 1998; ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef]
    [Google Scholar]
  12. Ibrahim, A., Gerner-Smidt, P. & Liesack, W. ( 1997; ). Phylogenetic relationship of the twenty-one DNA groups of the genus Acinetobacter as revealed by 16S ribosomal DNA sequence analysis. Int J Syst Bacteriol 47, 837–841.[CrossRef]
    [Google Scholar]
  13. Juni, E. ( 1972; ). Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus. J Bacteriol 112, 917–931.
    [Google Scholar]
  14. Juni, E. ( 1984; ). Genus III. Acinetobacter Brisou and Prévot 1954, 727AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 303–307. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  15. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  16. Nemec, A., Dijkshoorn, L. & Ježek, P. ( 2000; ). Recognition of two novel phenons of the genus Acinetobacter among non-glucose-acidifying isolates from human specimens. J Clin Microbiol 38, 3937–3941.
    [Google Scholar]
  17. Nemec, A., De Baere, T., Tjernberg, I., Vaneechoutte, M., van der Reijden, T. J. K. & Dijkshoorn, L. ( 2001; ). Acinetobacter ursingii sp. nov. and Acinetobacter schindleri sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 51, 1891–1899.[CrossRef]
    [Google Scholar]
  18. Tjernberg, I. & Ursing, J. ( 1989; ). Clinical strains of Acinetobacter classified by DNA-DNA hybridization. APMIS 97, 595–605.[CrossRef]
    [Google Scholar]
  19. Vaneechoutte, M., Tjernberg, I., Baldi, F., Pepi, M., Fani, R., Sullivan, E. R., van der Toorn, J. & Dijkshoorn, L. ( 1999; ). Oil-degrading Acinetobacter strain RAG-1 and strains described as ‘Acinetobacter venetianus sp. nov.’ belong to the same genomic species. Res Microbiol 150, 69–73.[CrossRef]
    [Google Scholar]
  20. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
  21. Wilson, K. ( 1987; ). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Green Publishing and Wiley-Interscience.
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