1887

Abstract

A coccoid- and amorphous-shaped, non-gliding, reddish bacterium, designated SG-29, was isolated from seawater in the western North Pacific Ocean near Japan. The strain was Gram-stain-negative, obligately aerobic, heterotrophic and catalase-positive. Nitrate was reduced to nitrogen and acid was produced from aesculin, turanose, 2-keto-gluconate and arabinose. Growth occurred with 1–5 % NaCl (optimum 2 % NaCl) and at 5–37 °C (optimum 20–30 °C). The G+C content of genomic DNA was 68.9 mol% (HPLC). The predominant isoprenoid quinone was MK-7 and the major cellular fatty acids (>10 %) were iso-Cω9, Cω8 and iso-C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SG-29 belonged to the phylum . The most closely related strain was JCM 9785, with which the isolate exhibited 86.1 % 16S rRNA gene sequence similarity. On the basis of phenotypic and phylogenetic features, strain SG-29 is classified in a novel genus and species within the family ‘’, for which the name gen. nov., sp. nov. ( = NBRC 107124  = KCTC 23197) is proposed.

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2011-09-01
2024-12-09
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References

  1. Alfredsson G. A., Kristjansson J. K., Hjorleifsdottir S., Stetter K. O. 1988; Rhodothermus marinus gen. nov., sp. nov., a thermophilic, halophilic bacterium from submarine hot springs in Iceland. J Gen Microbiol 134:299–306
    [Google Scholar]
  2. Antón J., Oren A., Benlloch S., Rodríguez-Valera F., Amann R., Rosselló-Mora R. 2002; Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds. Int J Syst Evol Microbiol 52:485–491[PubMed]
    [Google Scholar]
  3. Bardavid R. E., Ionescu D., Oren A., Rainey F. A., Hollen B. J., Bagaley D. R., Small A. M., McKay C. 2007; Selective enrichment, isolation and molecular detection of Salinibacter and related extremely halophilic Bacteria from hypersaline environments. Hydrobiologia 576:3–13 [View Article]
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  6. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  7. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–147 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  8. Ludwig W., Euzéby J., Whitman W. B. 2008; Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes.. http://www.bergeys.org/outlines/Bergeys_Vol_4_Outline.pdf
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  10. Marteinsson V. T., Bjornsdottir S. H., Bienvenu N., Jakob K., Kristjansson J. K., Birrien J.-L. 2010; Rhodothermus profundi sp. nov., a thermophilic bacterium isolated from a deep-sea hydrothermal vent in the Pacific Ocean. Int J Syst Evol Microbiol 60:2729–2734 [CrossRef]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  12. Murray R. G. E., Doetsch R. N., Robinow F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp. 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  14. Sako Y., Takai K., Ishida Y., Uchida A., Katayama Y. 1996; Rhodothermus obamensis sp. nov., a modern lineage of extremely thermophilic marine bacteria. Int J Syst Bacteriol 46:1099–1104 [View Article][PubMed]
    [Google Scholar]
  15. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc..
  16. Swofford D. L. 2002; paup*: Phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, MA: Sinauer Associates..
  17. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  19. Vaisman N., Oren A. 2009; Salisaeta longa gen. nov., sp. nov., a red, halophilic member of the Bacteroidetes . Int J Syst Evol Microbiol 59:2571–2574 [View Article][PubMed]
    [Google Scholar]
  20. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703[PubMed]
    [Google Scholar]
  21. Xie C. H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [View Article][PubMed]
    [Google Scholar]
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