Two strains of acid-neutralizing bacteria, E25T and E21, were isolated from torpedo grass (Panicum repens) growing in highly acidic swamps (pH 2–4) in actual acid sulfate soil areas of Thailand. Cells of the strains were Gram-negative, aerobic, non-spore-forming rods, 0.6–0.8 µm wide and 1.6–2.1 µm long. The strains showed good growth at pH 4.0–8.0 and 17–37 °C. The organisms contained ubiquinone Q-8 as the predominant isoprenoid quinone and C16 : 0, C17 : 0 cyclo and C18 : 1ω7c as the major fatty acids. Their fatty acid profiles were similar to those reported for other Burkholderia species. The DNA G+C content of the strains was 65 mol%. On the basis of 16S rRNA gene sequence similarity, the strains were shown to belong to the genus Burkholderia. Although the calculated 16S rRNA gene sequence similarity of E25T to strain E21 and the type strains of Burkholderiaunamae, B. tropica, B. sacchari, B. nodosa and B. mimosarum was 100, 98.7, 98.6, 97.6, 97.4 and 97.3 %, respectively, strains E25T and E21 formed a group that was distinct in the phylogenetic tree; the DNA–DNA relatedness of E25T to E21 and B. unamae CIP 107921T, B. tropica LMG 22274T, B. sacchari LMG 19450T, B. nodosa LMG 23741T and B. mimosarum LMG 23256T was 90, 42, 42, 42, 45 and 35 %, respectively. The results of physiological and biochemical tests including whole-cell protein pattern analysis allowed phenotypic differentiation of these strains from previously described Burkholderia species. Therefore, strains E25T and E21 represent a novel species, for which the name Burkholderia bannensis sp. nov. is proposed. The type strain is E25T ( = NBRC 103871T = BCC 36998T).
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Funding
This study was supported by the:
Institute for Fermentation (IFO; Osaka, Japan)
JST-NSFC Joint Research Program
Japan Society for Promotion of Science
(Award 20580365 and 21780300)
‘High-Tech Research Center Projects’ of the Ministry of Education, Culture, Sports, Science, and Technology of Japan
AizawaT.,
NguyenB. V.,
VijarnsornP.,
KimotoK.,
SasakiS.,
NakajimaM.,
SunairiM.2008; Application of symbiotic bacteria isolated from plants adapted to actual acid sulfate soil. In Development of New Bioremediation Systems of Acid Sulfate Soil for Agriculture and Forestry pp. 57–62 Edited by
SasakiS.,
IshiiR.,
HasegawaI.,
TokuyamaT.,
HanzawaK.,
SumidaH.,
UedaS.,
NoguchiA.,
MatsumotoR. et al.
Kyoto: Shoukadoh;
AizawaT.,
NguyenB. V.,
NakajimaM.,
SunairiM.2010b; Burkholderia heleia sp. nov., a nitrogen-fixing bacterium isolated from an aquatic plant, Eleocharis dulcis, that grows in highly acidic swamps in actual acid sulfate soil areas of Vietnam. Int J Syst Evol Microbiol 60:1152–1157 [View Article][PubMed]
BaldaniV. L. D.,
OliveiraE.,
BalotaE.,
BaldaniJ. I.,
KirchhofG.,
DöbereinerJ.1997; Burkholderia brasilensis sp. nov., uma nova espécie de bactéria diazotrófica endofitica. An Acad Bras Cienc 69:116 (in Portuguese)
BrämerC. O.,
VandammeP.,
da SilvaL. F.,
GomezJ. G. C.,
SteinbüchelA.2001; Burkholderia sacchari sp. nov., a polyhydroxyalkanoate-accumulating bacterium isolated from soil of a sugar-cane plantation in Brazil. Int J Syst Evol Microbiol 51:1709–1713[PubMed][CrossRef]
Caballero-MelladoJ.,
Onofre-LemusJ.,
Estrada-de Los SantosP.,
Martínez-AguilarL.2007; The tomato rhizosphere, an environment rich in nitrogen-fixing Burkholderia species with capabilities of interest for agriculture and bioremediation. Appl Environ Microbiol 73:5308–5319 [View Article][PubMed]
ChenW. M.,
JamesE. K.,
CoenyeT.,
ChouJ. H.,
BarriosE.,
de FariaS. M.,
ElliottG. N.,
SheuS. Y.,
SprentJ. I.,
VandammeP.2006; Burkholderia mimosarum sp. nov., isolated from root nodules of Mimosa spp. from Taiwan and South America. Int J Syst Evol Microbiol 56:1847–1851 [View Article][PubMed]
ChenW.-M.,
de FariaS. M.,
JamesE. K.,
ElliottG. N.,
LinK.-Y.,
ChouJ.-H.,
SheuS.-Y.,
CnockaertM.,
SprentJ. I.,
VandammeP.2007; Burkholderia nodosa sp. nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella
. Int J Syst Evol Microbiol 57:1055–1059 [View Article][PubMed]
ChunJ.,
LeeJ.-H.,
JungY.,
KimM.,
KimS.,
KimB. K.,
LimY.-W.2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261[CrossRef]
CoenyeT.,
VandammeP.2003; Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol 5:719–729 [View Article][PubMed]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
FelsensteinJ.2005; phylip (Phylogeny Inference Package) version 3.65. Distributed by the author. Department of Genome Sciences, University of Washington; Seattle, USA:
HashidokoY.,
TadaM.,
OsakiM.,
TaharaS.2002; Soft gel medium solidified with gellan gum for preliminary screening for root-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplane of plants. Biosci Biotechnol Biochem 66:2259–2263 [View Article][PubMed]
JukesT. H.,
CantorC. R.1969; Evolution of protein molecules. In Mammalian Protein Metabolismvol. 2 pp. 21–132 Edited by
MunroH. N.
New York: Academic Press;
KimotoK.,
AizawaT.,
UraiM.,
NguyenB. V.,
SuzukiK.,
NakajimaM.,
SunairiM.2010; Acidocella aluminiidurans sp. nov., an aluminium-tolerant bacterium isolated from Panicum repens grown in a highly acidic swamp in actual acid sulfate soil area of Vietnam. Int J Syst Evol Microbiol 60:764–768 [View Article][PubMed]
PerinL.,
Martínez-AguilarL.,
Paredes-ValdezG.,
BaldaniJ. I.,
Estrada-de Los SantosP.,
ReisV. M.,
Caballero-MelladoJ.2006; Burkholderia silvatlantica sp. nov., a diazotrophic bacterium associated with sugar cane and maize. Int J Syst Evol Microbiol 56:1931–1937 [View Article][PubMed]
PotB.,
VandammeP.,
KerstersK.1994; Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods (Chemical Methods Prokaryotic Systematics Series) pp. 493–521 Edited by
GoodfellowM.,
O’DonnellA. G.
Chichester: Wiley;
SasakiS.,
IshiiR.,
HasegawaI.,
TokuyamaT.,
HanzawaK.,
SumidaH.,
UedaS.,
NoguchiA.,,
MatsumotoR. et al.2008Development of New Bioremediation Systems of Acid Sulfate Soil for Agriculture and Forestry Kyoto: Shoukadoh;
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
StackebrandtE.,
GoebelB.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
TamuraT.,
HatanoK.2001; Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanesminutisporangius Ruan et al. 1986 and ‘Actinoplanesaurantiacus’ to Cryptosporangiumminutisporangium comb. nov. and Cryptosporangiumaurantiacum sp. nov.. Int J Syst Evol Microbiol 51:2119–2125[PubMed][CrossRef]
TamuraT.,
NakagaitoY.,
NishiiT.,
HasegawaT.,
StackebrandtE.,
YokotaA.1994; A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov.. Int J Syst Bacteriol 44:193–203 [View Article][PubMed]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E. et al.1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
WeberO. B.,
BaldaniV. L. D.,
TeixeiraK. R. S.,
KirchhofG.,
BaldaniJ. I.,
DöbereinerJ.1999; Isolation and characterization of diazotrophic bacteria from banana and pineapple plants. Plant Soil 210:103–113 [View Article]
YabuuchiE.,
KosakoY.,
OyaizuH.,
YanoI.,
HottaH.,
HashimotoY.,
EzakiT.,
ArakawaM.1992; Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov.. Microbiol Immunol 36:1251–1275[PubMed][CrossRef]
YamadaY.,
Takinami-NakamuraH.,
TaharaY.,
OyaizuH.,
KomagataK.1982; The ubiquinone systems in the strains of Pseudomonas species. J Gen Appl Microbiol 28:7–12 [View Article]