sp. nov., an acid-neutralizing bacterium isolated from torpedo grass () growing in highly acidic swamps Free

Abstract

Two strains of acid-neutralizing bacteria, E25 and E21, were isolated from torpedo grass () growing in highly acidic swamps (pH 2–4) in actual acid sulfate soil areas of Thailand. Cells of the strains were Gram-negative, aerobic, non-spore-forming rods, 0.6–0.8 µm wide and 1.6–2.1 µm long. The strains showed good growth at pH 4.0–8.0 and 17–37 °C. The organisms contained ubiquinone Q-8 as the predominant isoprenoid quinone and C, C cyclo and Cω7 as the major fatty acids. Their fatty acid profiles were similar to those reported for other species. The DNA G+C content of the strains was 65 mol%. On the basis of 16S rRNA gene sequence similarity, the strains were shown to belong to the genus Although the calculated 16S rRNA gene sequence similarity of E25 to strain E21 and the type strains of , , , and was 100, 98.7, 98.6, 97.6, 97.4 and 97.3 %, respectively, strains E25 and E21 formed a group that was distinct in the phylogenetic tree; the DNA–DNA relatedness of E25 to E21 and CIP 107921, LMG 22274, LMG 19450, LMG 23741 and LMG 23256 was 90, 42, 42, 42, 45 and 35 %, respectively. The results of physiological and biochemical tests including whole-cell protein pattern analysis allowed phenotypic differentiation of these strains from previously described species. Therefore, strains E25 and E21 represent a novel species, for which the name sp. nov. is proposed. The type strain is E25 ( = NBRC 103871  = BCC 36998).

Funding
This study was supported by the:
  • Institute for Fermentation (IFO; Osaka, Japan)
  • JST-NSFC Joint Research Program
  • Japan Society for Promotion of Science (Award 20580365 and 21780300)
  • ‘High-Tech Research Center Projects’ of the Ministry of Education, Culture, Sports, Science, and Technology of Japan
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2011-07-01
2024-03-29
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