1887

Abstract

A Gram-stain-positive, ovoid to short rod-shaped, yellow-pigmented bacterium, designated strain DY66, was isolated from tidal-flat sediment collected from Deukryang Bay (Republic of Korea), and its taxonomic position was investigated by using a polyphasic approach. Strain DY66 grew optimally at 30 °C and pH 8–9 and in 2 % (w/v) NaCl. The peptidoglycan type was A4α, -Lys–-Ala–-Glu, and tyvelose and glucose were the major cell-wall sugars. The predominant menaquinones were MK-10 and MK-9. Major cellular fatty acids (>10 % of total) were anteiso-C and iso-C. The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unknown aminophospholipid and three unknown aminolipids. The DNA G+C content was 59.1 mol%. This chemotaxonomic profile supported the assignment of strain DY66 to the genus . Phylogenetic analysis based on 16S rRNA gene sequences also indicated that strain DY66 belonged to the family and was related to the genus . On the basis of the evidence presented in this study, strain DY66 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain DY66 ( = KCTC 19557  = JCM 16364).

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2011-07-01
2019-10-14
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Maximum-likelihood (Fig. S1) and maximum-parsimony (Fig. S2) trees based on nearly complete 16S rRNA gene sequences showing relationships between strain DY66 and closely related recognized species. Bars, 0.01 substitutions per nucleotide position (Fig. S1) and 5 changes (Fig. S2). [PDF file of Supplementary Figs S1 and S2](44 KB)

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Scanning electron micrograph of cells of strain DY66 . Cells were grown on TSA at 30 °C for 3 days. Bar, 3 µm.

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TLC of cell-wall peptidoglycan hydrolysate of strain DY66 . (a) Total hydrolysate (4 M HCl, 16 h, 100 °C) of the peptidoglycan, containing the amino acids lysine, glutamic acid and alanine. (b) Partial peptidoglycan hydrolysate (4 M HCl, 0.75 h, 100 °C) containing (in addition to the amino acids) the peptides L-Ala→D-Glu (1), L-Lys←L-Ala (2), L-Lys→D-Ala (3) and L-Ala→L-Lys→D-Ala (4).

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Cell-wall sugar profile of strain DY66 . ZA, YIM 90734 ; ZH, YIM 70185 . Glc, Gal, Man, Rha and Mixture are standards containing the indicated sugars [Glc, glucose; Gal, galactose; Man, mannose; Rha, rhamnose; Mixture (Rib, ribose; Xyl, xylose; Ara, arabinose)].

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Polar lipid profile of strain DY66 after two-dimensional TLC and detection with Zinzadze reagent. DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PC, phosphatidylcholine; AL1–3, unknown aminolipids; APL, unknown aminophospholipid. Authentic standards (Sigma): PE*, phosphatidylethanolamine; PI*, phosphatidylinositol.

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