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Volume 53, Issue 5

gen. nov., sp. nov., isolated from a thermophilic aerobic digester of municipal sludge Free

Abstract

A bacterial strain, N2-680 (=DSM 15264=LMG 21760), isolated from a thermophilic aerobic digester of municipal sludge, was characterized with respect to its morphology, physiology and taxonomy. Phenotypically, the isolate was a Gram-negative rod with a polar flagellum, catalase- and oxidase-positive, containing cytoplasmic inclusions of poly--hydroxybutyrate and had an optimal growth temperature of about 47 °C. Strain N2-680 was unable to reduce nitrate and could use organic acids, amino acids and carbohydrates as single carbon sources. Chemotaxonomic analysis revealed that ubiquinone 8 was the major respiratory quinone of this organism and that phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. At 50 °C, the major components in fatty acid methyl ester analysis were C and cyclo-C. The highest 16S rDNA sequence identity of isolate N2-680 was to and (95·7 %) and to and (95·6 %). 16S rDNA sequence similarities to species of two related thermophilic genera, and , were lower (93·6 and 94·7 %). On the basis of phylogenetic analyses and physiological and chemotaxonomic characteristics, it is proposed that isolate N2-680 represents a new genus and species, for which the name gen. nov., sp. nov. is proposed.

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Keyword(s): PHB, poly-β-hydroxybutyrate
SGM
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2003-09-01
2024-04-16
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Caenibacterium thermophilum gen. nov., sp. nov., isolated from a thermophilic aerobic digester of municipal sludge
Int J Syst Evol Microbiol 53, 1375 (2003); https://doi.org/10.1099/ijs.0.02622-0
/content/journal/ijsem/10.1099/ijs.0.02622-0
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