1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 53, Issue 5

gen. nov., sp. nov., isolated from a thermophilic aerobic digester of municipal sludge Free

Abstract

A bacterial strain, N2-680 (=DSM 15264=LMG 21760), isolated from a thermophilic aerobic digester of municipal sludge, was characterized with respect to its morphology, physiology and taxonomy. Phenotypically, the isolate was a Gram-negative rod with a polar flagellum, catalase- and oxidase-positive, containing cytoplasmic inclusions of poly--hydroxybutyrate and had an optimal growth temperature of about 47 °C. Strain N2-680 was unable to reduce nitrate and could use organic acids, amino acids and carbohydrates as single carbon sources. Chemotaxonomic analysis revealed that ubiquinone 8 was the major respiratory quinone of this organism and that phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. At 50 °C, the major components in fatty acid methyl ester analysis were C and cyclo-C. The highest 16S rDNA sequence identity of isolate N2-680 was to and (95·7 %) and to and (95·6 %). 16S rDNA sequence similarities to species of two related thermophilic genera, and , were lower (93·6 and 94·7 %). On the basis of phylogenetic analyses and physiological and chemotaxonomic characteristics, it is proposed that isolate N2-680 represents a new genus and species, for which the name gen. nov., sp. nov. is proposed.

  • Published Online:
Keyword(s): PHB, poly-β-hydroxybutyrate
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02622-0
2003-09-01
2023-06-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/5/ijs531375.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02622-0&mimeType=html&fmt=ahah

References

  1. Aragno M., Schlegel H. G. 1992; The mesophilic hydrogen oxidizing (Knallgas) bacteria. In The Prokaryotes , 2nd edn. pp 3917–3933Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
     [Google Scholar]
  2. Busse H. J., Auling G. 1992; The genera Alcaligenes and ‘ Achromobacter ’. In The Prokaryotes , 2nd edn. pp 2544–2555Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
     [Google Scholar]
  3. Busse H.-J., Kämpfer P., Moore E. R. B.7 other authors 2002; Thermomonas haemolytica gen. nov., sp. nov. a γ -proteobacterium from kaolin slurry. Int J Syst Evol Microbiol 52:473–483
     [Google Scholar]
  4. Carlton B. C., Brown B. J. 1981; Gene mutation. In Manual of Methods for General Bacteriology pp 409–443Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
     [Google Scholar]
  6. Doetsch R. N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp 21–33Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  7. Felsenstein J. 1989; phylip – phylogeny inference package. Cladistics 5:164–166
     [Google Scholar]
  8. Heimbrook M. E., Wang W. L. L., Campbell G. 1989; Staining bacterial flagella easily. J Clin Microbiol 27:2612–2615
     [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp 21–132Edited by Munro H. N. New York: Academic Press;
     [Google Scholar]
  10. Kalmbach S., Manz W., Wecke J., Szewzyk U. 1999; Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system. Int J Syst Bacteriol 49:769–777 [CrossRef]
     [Google Scholar]
  11. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [CrossRef]
     [Google Scholar]
  12. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
     [Google Scholar]
  13. Malmqvist A., Welander T., Moore E., Ternström A., Molin G., Sternström I. 1994; Ideonella dechloratans , gen. nov., sp. nov. a new bacterium capable of growing anaerobically with chlorate as an electron acceptor. Syst Appl Microbiol 17:58–64 [CrossRef]
     [Google Scholar]
  14. Manaia C. M., Moore E. R. B. 2002; Pseudomonas thermotolerans sp. nov., a thermotolerant species of the genus Pseudomonas sensu stricto . Int J Syst Evol Microbiol 52:2203–2209 [CrossRef]
     [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  16. Moreira C., Rainey F. A., Nobre M. F., da Silva M. T., da Costa M. S. 2000; Tepidimonas ignava gen. nov., sp. nov. a new chemolithohererotrophic and slightly thermophilic member of the β - Proteobacteria . Int J Syst Evol Microbiol 50:735–742 [CrossRef]
     [Google Scholar]
  17. Mulder E. G. 1989; Genus Leptothrix Kützing 1843, 198AL. In Bergey's Manual of Systematic Bacteriology vol 3 pp 1998–2003Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams &Wilkins;
     [Google Scholar]
  18. Mulder E. G., Deinema M. H. 1992; The sheathed bacteria. In The Prokaryotes , 2nd edn. pp 2612–2624Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
     [Google Scholar]
  19. Nealson K. H. 1992; The manganese-oxidizing bacteria. In The Prokaryotes , 2nd edn. pp 2310–2320Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
     [Google Scholar]
  20. Nogales B., Moore E. R. B., Llobet-Brossa E., Rossello-Mora R., Amann R., Timmis K. N. 2001; Combined use of 16S ribosomal DNA and 16S rRNA to study the bacterial community of polychlorinated biphenyl-polluted soil. Appl Environ Microbiol 67:1874–1884 [CrossRef]
     [Google Scholar]
  21. Palleroni N. J., Palleroni A. V. 1978; Alcaligenes latus , a new species of hydrogen-utilizing bacteria. Int J Syst Bacteriol 28:416–424 [CrossRef]
     [Google Scholar]
  22. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
     [Google Scholar]
  23. Pfennig N. 1978; Rhodocyclus purpureus gen. nov. and sp. nov., a ring-shaped, vitamin B12-requiring member of the family Rhodospirillaceae . Int J Syst Bacteriol 28:283–288 [CrossRef]
     [Google Scholar]
  24. Prado A., da Costa M. S., Madeira V. M. C. 1988; Effect of growth temperature on the lipid composition of two strains of Thermus sp. J Gen Microbiol 134:1653–1660
     [Google Scholar]
  25. Siefert E., Irgens R. L., Pfennig N. 1978; Phototrophic purple and green bacteria in a sewage treatment plant. Appl Environ Microbiol 35:38–44
     [Google Scholar]
  26. Siering P. L., Ghiorse W. C. 1996; Phylogeny of the Sphaerotilus-Leptothrix group inferred from morphological comparisons, genomic fingerprinting, and 16S ribosomal DNA sequence analyses. Int J Syst Bacteriol 46:173–182 [CrossRef]
     [Google Scholar]
  27. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Bacteriology pp 409–443Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  28. Spring S., Kämpfer P., Ludwig W., Schleifer K. H. 1996; Polyphasic characterization of the genus Leptothrix : new descriptions of Leptothrix mobilis sp. nov. and Leptothrix discophora sp. nov. nom. rev. and emended description of Leptothrix cholodnii emend. Syst Appl Microbiol 19634–643 [CrossRef]
     [Google Scholar]
  29. Stöhr R., Waberski A., Liesack W., Völker H., Wehmeyer U., Thomm M. 2001; Hydrogenophilus hirschii sp. nov., a novel thermophilic hydrogen-oxidizing β -proteobacterium isolated from Yellowstone National Park. Int J Syst Evol Microbiol 51:481–488
     [Google Scholar]
  30. Suyama T., Hosoya H., Tokiwa Y. 1998; Bacterial isolates degrading aliphatic polycarbonates. FEMS Microbiol Lett 161:255–261 [CrossRef]
     [Google Scholar]
  31. Suyama T., Shigematsu T., Takaichi S., Nodasaka Y., Fujikawa S., Hosoya H., Tokiwa Y., Kanagawa T., Hanada S. 1999; Roseateles depolymerans gen. nov., sp. nov. a new bacteriochlorophyll a -containing obligate aerobe belonging to the β -subclass of the Proteobacteria . Int J Syst Bacteriol 49:449–457 [CrossRef]
     [Google Scholar]
  32. Suzuki K., Goodfellow M., O'Donnell A. G. 1993; Cell envelopes and classification. In Handbook of New Bacterial Systematics pp 195–250Edited by Goodfellow M., O'Donnell A. G. London: Academic Press;
     [Google Scholar]
  33. Takeda M., Koizumi J., Yabe K., Adachi K. 1998; Thermostable poly(3-hydroxybutyrate) depolymerase of a thermophilic strain of Leptothrix sp. isolated from a hot spring. J Ferment Bioeng 85:375–380 [CrossRef]
     [Google Scholar]
  34. Takeda M., Kamagata Y., Ghiorse W. C., Hanada S., Koizumi J. 2002; Caldimonas manganoxidans gen. nov., sp nov., a poly(3-hydroxybutyrate)-degrading, manganese-oxidizing thermophile. Int J Syst Evol Microbiol 52:895–900 [CrossRef]
     [Google Scholar]
  35. Tindall B. J. 1989; Fully saturated menaquinones in the archaebacteriun Pyrobaculum islandicum . FEMS Microbiol Lett 60:251–254 [CrossRef]
     [Google Scholar]
  36. Wilkinson S. G. 1988; Gram-negative bacteria. In Microbial Lipids vol 1 pp 299–488Edited by Ratledge C., Wilkinson S. G. London: Academic Press;
     [Google Scholar]
  37. Willems A., Gillis M., De Ley J. 1991; Transfer of Rhodocyclus gelatinosus to Rubrivivax gelatinosus gen. nov., comb. nov. and phylogenetic relationships with Leptothrix , Sphaerotilus natans , Pseudomonas saccharophila , and Alcaligenes latus . Int J Syst Bacteriol 41:65–73 [CrossRef]
     [Google Scholar]
  38. Yu Z., Mohn W. W. 1999; Isolation and characterization of thermophilic bacteria capable of degrading dehydroabietic acid. Can J Microbiol 45:513–519 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02622-0
Loading
Caenibacterium thermophilum gen. nov., sp. nov., isolated from a thermophilic aerobic digester of municipal sludge
Int J Syst Evol Microbiol 53, 1375 (2003); https://doi.org/10.1099/ijs.0.02622-0
/content/journal/ijsem/10.1099/ijs.0.02622-0
/content/journal/ijsem/10.1099/ijs.0.02622-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error