1887

Abstract

A novel, Gram-reaction-positive, aerobic, rod-shaped, non-motile bacterial strain, designated NAL101, was isolated from gajami-sikhae, a traditional Korean fermented seafood made of flatfish. Growth occurred at 4–45 °C, at pH 5–10 and in 0–12 % (w/v) NaCl. Optimum growth occurred at 30–37 °C, at pH 8 and in 0–1 % (w/v) NaCl. The cell-wall amino acids were 2,4-diaminobutyric acid, alanine, glycine, threonine and glutamic acid and the major fatty acids were anteiso-C, iso-C and anteiso-C. The predominant menaquinone was MK-11. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The 16S rRNA gene sequence of strain NAL101 showed 97.7 % similarity to that of MM2LB, its closest relative. The DNA G+C content was 68.8 mol% and DNA–DNA hybridization values with closely related strains were <22 %. Phylogenetic analyses based on 16S rRNA gene sequences as well as differences in its physiological and biochemical characteristics indicated that strain NAL101 represents a novel species of the genus in the family , for which the name sp. nov. is proposed. The type strain is NAL101 ( = KACC 14220  = JCM 16465).

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2011-10-01
2019-11-17
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vol. , part 10, pp. 2353 - 2357

Two-dimensional TLC of polar lipids of strain NAL101 on silica gel sheets. Phospholipids, glycolipids, and total lipid were detected with zinzadze reagent (a), α-naphthol reagent (b) and 10% ethanolic molybdatophosphoric acid reagent (c), respectively. PG, phosphatidylglycerol; DPG, diphosphatidylglycerol; GL, glycolipid.



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