1887

Abstract

(basonym, ) is an extremely halophilic, aerobic archaeon isolated from saline soils, which was described on the basis of phenotypic features of several strains. The designated type strain of the species (1m=VKM B-1733=JCM 9100) was shown recently to differ from the other strains. In this study, isolates have been characterized with regard to phenotypic features, polar lipid content, comparison of 16S rRNA gene sequences and DNA–DNA hybridization. On the basis of these data, a novel species that includes the other isolates is proposed, with the name sp. nov. The type strain of this novel species is 4p (=VKM B-1739=JCM 10247). The DNA G+C content of this novel species is 64·2–64·9 mol% (64·4 mol% for the type strain).

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2004-03-01
2019-10-23
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References

  1. De Ley, J. & Tijtgat, R. ( 1970; ). Evaluation of membrane filter methods for DNA–DNA hybridization. Antonie van Leeuwenhoek 36, 461–474.[CrossRef]
    [Google Scholar]
  2. Ferragut, C. & LeClerc, H. ( 1976; ). Étude comparative des méthodes de détermination du T m de l'ADN bactérien. Ann Microbiol (Paris) 127A, 223–235 (in French).
    [Google Scholar]
  3. Grant, W. D., Kamekura, M., McGenity, T. J. & Ventosa, A. ( 2001; ). Class III. Halobacteria class. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 294–301. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  4. Johnson, J. L. ( 1994; ). Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology, pp. 655–682. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  5. Kamekura, M. ( 1993; ). Lipids of extreme halophiles. In The Biology of Halophilic Bacteria, pp. 135–161. Edited by R. H. Vreeland & L. I. Hochstein. Boca Raton, FL: CRC Press.
  6. Kamekura, M. & Dyall-Smith, M. L. ( 1995; ). Taxonomy of the family Halobacteriaceae and the description of two new genera Halorubrobacterium and Natrialba. J Gen Appl Microbiol 41, 333–350.[CrossRef]
    [Google Scholar]
  7. Kamekura, M., Mizuki, T., Usami, R., Yoshida, Y., Horikoshi, K. & Vreeland, R. H. ( 2004; ). The potential utility of signature bases in the 16S rRNA gene sequences to aid in the definition of genera of halobacteria. In Halophilic Microorganisms, pp. 77–87. Edited by A. Ventosa. New York: Springer.
  8. Lapage, S. P., Sneath, P. H. A., Lessel, E. F., Skerman, V. B. D., Seeliger, H. P. R. & Clark, W. A. (editors) ( 1992; ). International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code. Washington, DC: American Society for Microbiology.
  9. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  10. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  11. McGenity, T. J. & Grant, W. D. ( 1995; ). Transfer of Halobacterium saccharovorum, Halobacterium sodomense, Halobacterium trapanicum NRC 34021 and Halobacterium lacusprofundi to the genus Halorubrum gen. nov., as Halorubrum saccharovorum comb. nov., Halorubrum sodomense comb. nov., Halorubrum trapanicum comb. nov., and Halorubrum lacusprofundi comb. nov. Syst Appl Microbiol 18, 237–243.[CrossRef]
    [Google Scholar]
  12. McGenity, T. J. & Grant, W. D. ( 2001; ). Genus VII. Halorubrum. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 320–324. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  13. Oren, A. & Ventosa, A. ( 1996; ). A proposal for the transfer of Halorubrobacterium distributum and Halorubrobacterium coriense to the genus Halorubrum as Halorubrum distributum comb. nov. and Halorubrum coriense comb. nov., respectively. Int J Syst Bacteriol 46, 1180.[CrossRef]
    [Google Scholar]
  14. Oren, A., Kamekura, M. & Ventosa, A. ( 1997a; ). Confirmation of strain VKM B-1733 as the type strain of Halorubrum distributum. Int J Syst Bacteriol 47, 231–232.[CrossRef]
    [Google Scholar]
  15. Oren, A., Ventosa, A. & Grant, W. D. ( 1997b; ). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47, 233–238.[CrossRef]
    [Google Scholar]
  16. Owen, R. J. & Hill, L. R. ( 1979; ). The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists, pp. 277–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  17. Owen, R. J. & Pitcher, D. ( 1985; ). Current methods for estimating DNA base composition and levels of DNA–DNA hybridization. In Chemical Methods in Bacterial Systematics, pp. 67–93. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  18. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  19. Torreblanca, M., Rodriguez-Valera, F., Juez, G., Ventosa, A., Kamekura, M. & Kates, M. ( 1986; ). Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition, and description of Haloarcula gen. nov. and Haloferax gen. nov. Syst Appl Microbiol 8, 89–99.[CrossRef]
    [Google Scholar]
  20. Zvyagintseva, I. S. & Tarasov, A. L. ( 1987; ). Extreme halophilic bacteria from saline soils. Mikrobiologiya 56, 839–844 (in Russian).
    [Google Scholar]
  21. Zvyagintseva, I. S. & Tarasov, A. L. ( 1989; ). Halobacterium distributus sp. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 31. Int J Syst Bacteriol 39, 495–497; erratum 40, 106.
    [Google Scholar]
  22. Zvyagintseva, I. S., Kudryashova, E. B. & Bulygina, E. S. ( 1996; ). Proposal of a new type strain of Halobacterium distributum. Microbiology (English translation of Mikrobiologiya) 65, 352–354.
    [Google Scholar]
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