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Abstract

A Gram-reaction-positive, motile, coccus-shaped actinobacterium, designated strain T2A-S27, was isolated from a roof tile in Oporto (Portugal) and studied using a polyphasic approach. The 16S rRNA gene sequence of the novel isolate showed high similarity to that of KST3-3 (97.8 % sequence similarity). Strain T2A-S27 showed lower 16S rRNA gene sequence similarities with other members of the genus and members of the family (<94 %). A phylogenetic tree, based on 16S rRNA gene sequences, showed that strain T2A-S27 formed a coherent clade with the type strain of and . The isolate was characterized by the presence of diaminopimelic acid in the cell-wall peptidoglycan, MK-9(H) as the predominant menaquinone and a polar lipid profile consisting of diphosphatidylglycerol and phosphatidylglycerol. The fatty acid profile was dominated by anteiso-C. The DNA G+C content was 76.9 mol%. The low level of DNA–DNA relatedness to (46–47 %) and the results of the chemotaxonomic and physiological studies clearly distinguished strain T2A-S27 from recognized species of the genus . On the basis of its phylogenetic position and phenotypic traits, strain T2A-S27 ( = LMG 24148  = CECT 7306  = DSM 23768) represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The misclassified species is transferred to the new genus as comb. nov. The type strain of is KST3-3 ( = KCCM 42250  = NRRL B-24439).

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2011-10-01
2019-12-15
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vol. , part 10, pp. 2515 - 2519

Fatty acid composition of strain T2A-S27 and . [PDF](49KB)



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