1887

Abstract

Fourteen apiculate yeast strains isolated from various sources in South Africa, North America and the Hawaiian islands were found to be genetically divergent from other species by using randomly amplified polymorphic DNA (RAPD)-PCR. After cluster analysis of the RAPD-PCR fingerprints, five groups were recognized. DNA reassociation values among representatives of these groups and strains of species revealed that the strains represent five novel species. Four are described here as novel species of : sp. nov. (type CBS 8734), sp. nov. (type CBS 8821), sp. nov. (type CBS 8818) and sp. nov. (type CBS 8733). The fifth novel species, which is represented by only a single strain, CBS 8772, is not introduced as a new taxon. Phylogenetic analyses of the D1/D2 region of the 26S rDNA and internal transcribed spacer (ITS) regions with 5·8S rDNA sequences placed , , , and strain CBS 8772 close to and . The key characteristics for standard physiological identification of and were respectively maximal growth temperature and assimilation of 2-keto--gluconate. However, physiological characteristics did not allow the distinction of and strain CBS 8772 from or from . These three novel taxa can be identified by either ITS sequencing or PCR-RFLP of ITS regions using restriction enzymes II and fI.

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2003-09-01
2020-01-28
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DNA–DNA relatedness among some strains of the genera and . [PDF](125 KB)

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Phylogenetic positions of four novel species of and CBS 8772 based on the D1/D2 region of 26S rDNA. The tree was constructed by neighbour-joining analysis. Bootstrap percentages above 50 % from 1000 bootstrap replicates are shown. Names in bold correspond to sequences determined in this study. The outgroup species was . Bar, 1 % sequence divergence. [PDF](243 KB)

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Molecular identification scheme for separation of physiologically indistinguishable and species by PCR-RFLP of ITS regions. Restriction enzyme II separates from or from ; fI separates CBS 8772 from and or from ; and I separates from . Sizes of restriction fragments are shown. Numbers in bold indicate double bands. [PDF](838 KB)

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