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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 61, Issue 9

gen. nov., sp. nov. and sp. nov., gammaproteobacteria respectively isolated from coastal attached (fast) ice and surface seawater of the Antarctic Free

Abstract

Two Gram-negative, motile, aerobic, catalase- and oxidase-positive, rod-shaped strains, designated ZS5-23 and ZS6-22, were respectively isolated from Antarctic coastal attached (fast) ice and surface seawater samples. Both strains could grow at 4–35 °C (optimum 30 °C) and in the absence of NaCl. Analyses of 16S rRNA gene sequences revealed that strains ZS5-23 and ZS6-22 were closely related to each other (99.0 % sequence similarity) and belonged to the class , with their closest relatives being and species (93.1–94.3 % sequence similarity). The predominant cellular fatty acids in both strains were Cω8, C and summed feature 3 (Cω7 and/or iso-C 2-OH). Genomic DNA G+C contents of strains ZS5-23 and ZS6-22 were 51.5 and 51.8 mol%, respectively. The DNA–DNA relatedness between strains ZS5-23 and ZS6-22 was 50.9 %. Strains ZS5-23 and ZS6-22 could be differentiated from each other and from and species by differences in a number of phenotypic properties. Based on the data presented, strains ZS5-23 and ZS6-22 represent two novel species in a new genus in the class , for which the names gen. nov., sp. nov. (the type species) and sp. nov. are proposed. The type strain of is ZS5-23 ( = KACC 14066  = CCTCC AB 209246) and that of is ZS6-22 ( = KACC 14532  = CCTCC AB 209247).

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Funding
This study was supported by the:
  • Hi-Tech Research and Development Program of China (Award 2007AA021306 and 2007AA091903)
  • National Natural Science Foundation of China (Award 30500001 and 40706001)
  • COMRA Program (Award DYXM-115-02-2-6)
© 2011 IUMS
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2011-09-01
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Zhongshania antarctica gen. nov., sp. nov. and Zhongshania guokunii sp. nov., gammaproteobacteria respectively isolated from coastal attached (fast) ice and surface seawater of the Antarctic
Int J Syst Evol Microbiol 61, 2052 (2011); https://doi.org/10.1099/ijs.0.026153-0
/content/journal/ijsem/10.1099/ijs.0.026153-0
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