1887

Abstract

Five isolates, designated TA2, TA4, TA25, KOx and NS15 were isolated in previous studies by enrichment in mineral medium with potassium oxalate as the sole carbon source and were characterized using a polyphasic approach. The isolates were Gram-reaction-negative, aerobic, non-spore-forming rods. Phylogenetic analyses based on 16S rRNA and DNA gyrase B subunit () gene sequences confirmed that the isolates belonged to the genus and were most closely related to and (97.2–99.7 % 16S rRNA gene sequence similarity). The isolates could be differentiated from their closest relatives on the basis of several phenotypic characteristics. The major cellular fatty acid profiles of the isolates comprised C, Cω7, C cyclo and summed feature 3 (Cω7 and/or iso-C 2-OH). On the basis of DNA–DNA hybridization studies and phylogenetic analyses, the isolates represent three novel species within the genus , for which the names sp. nov. (TA25  = NBRC 106091  = CCM 7677  = DSM 23570), sp. nov. (KOx  = NBRC 106092  = CCM 2766  = DSM 23572) and sp. nov. (NS15  = NBRC 106088  = CCM 7667  = DSM 23571) are proposed.

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2011-09-01
2019-10-19
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Supplements

vol. , part 9, pp. 2247 - 2253.

Comparison of 16S rRNA and gene sequences of the new strains with strains of neighbouring species.

Similarities of and 16S rRNA gene sequences and DNA-DNA reassociation values between newly proposed type strains and phylogenetically related type strains of species.

Dendrogram based on cluster analysis of RI ribotype patterns obtained by automated ribotyping with the RiboPrinter microbial characterization system.

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