1887

Abstract

The actinomycete strains YIM 90001 and YIM 90005 were isolated on starch-casein agar [20 % (w/v) NaCl, pH 7·0] from a soil sample collected from Xinjiang Province in the west of China. The two isolates were aerobic and Gram-positive. The substrate mycelium was fragmented and an aerial mycelium was well developed on Czapek medium. The aerial mycelium formed long spore chains with branched short or long spore chains at maturity, which were straight to flexuous, and spores were non-motile. Based on the results of metabolic, chemotaxonomic and molecular analyses, the two isolates were identified as two new species of the genus , for which the names for strain YIM 90001 (=DSM 44617=CCTCC AA001015) and for strain YIM 90005 (=DSM 44590=CCTCC AA001016) are proposed.

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2003-09-01
2024-10-11
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Cui X. L., Mao P. H., Tseng M., Li W. J., Zhang L. P., Xu L. H., Jiang C. L. 2001; Streptomonospora salina gen. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51:357–363
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Conference limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789 [CrossRef]
    [Google Scholar]
  5. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [CrossRef]
    [Google Scholar]
  6. Huss V. A. R., Festl H., Schleifer K.-H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  7. Jahnke K.-D. 1992; basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73 [CrossRef]
    [Google Scholar]
  8. Kelly K. L. 1964 Inter-Society Color Council–National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  9. Kim S. B., Goodfellow M. 1999; Reclassification of Amycolatopsis rugosa Lechevalier et al. 1986 as Prauserella rugosa gen. nov., comb. nov. Int J Syst Bacteriol 49:507–512 [CrossRef]
    [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  11. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  12. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger as stationary phases. J Liquid Chromatogr 5:2359–2387 [CrossRef]
    [Google Scholar]
  13. Kroppenstedt R. M., Korn-Wendisch F., Fowler V. J., Stackebrandt E. 1981; Biochemical and molecular genetic evidence for transfer of Actinoplanes armeniacus into the family Streptomycetaceae . Zentbl Bakteriol Mikrobiol Hyg 1 Abt Orig C 2254–262
    [Google Scholar]
  14. Lechevalier M. P., Prauser H., Labeda D., Ruan J. S. 1986; Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int J Syst Bacteriol 36:29–37 [CrossRef]
    [Google Scholar]
  15. Locci R. 1989; Streptomyces and related genera. In Bergey's Manual of Systematic Bacteriology Vol 4 pp 2463–2468Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  17. Marmur J., Doty P. 1962; Determination of base composition of deoxyribonucleic acid from its denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  18. Mertz F. P., Yao R. C. 1993; Amycolatopsis alba sp. nov., isolated from soil. Int J Syst Bacteriol 43:715–720 [CrossRef]
    [Google Scholar]
  19. Miller L. T. 1982; A single derivatization method for bacterial fatty acid methyl esters including hydroxy acids. J Clin Microbiol 16:584–586
    [Google Scholar]
  20. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:1–6
    [Google Scholar]
  23. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  24. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system of Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  25. Stanek J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R.9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  28. Yassin A. F., Haggenei B., Budzikiewicz H., Schaal K. P. 1993; Fatty acid and polar lipid composition of the genus Amycolatopsis : Application of fast atom bombardment-mass spectrometry to structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43:414–420 [CrossRef]
    [Google Scholar]
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