1887

Abstract

In order to clarify the taxonomic position of , which shares 99·9 % 16S rDNA sequence identity (1433 of 1435 bp) with , we compared the two species in a polyphasic taxonomic approach. Results of 122 physiological and biochemical tests for DSM 13638 and 6a3 were identical, except for the lack of growth of 6a3 with perchlorate as the terminal electron acceptor. Presence of a gene and nitrogenase activity, a key feature of , were also detected in by Southern hybridization and by the acetylene reduction assay, respectively. Whole-cell SDS-PAGE profiles of SDS-soluble proteins of strains DSM 13638 and 6a3 were almost identical. DNA–DNA hybridization studies showed more than 90 % binding between and two strains of . These data provide evidence that the two bacteria belong to the same species and that is a later subjective synonym of .

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2003-07-01
2019-10-20
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