1887

Abstract

A novel Gram-negative, motile, bacteriochlorophyll -containing purple non-sulfur bacterium, strain JA248, was isolated from phototrophic enrichments of a yellow–green epilithic biofilm sample collected from Gulmarg, India. The genomic DNA GC content of strain JA248 was 63.8 mol%. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain JA248 had highest similarity to members of the genus and was closely related to DSM 729 (98.5 % sequence similarity) and DSM 133 (98.4 %) of the class . Strain JA248 was characterized based on polyphasic taxonomy, and distinct phenotypic and molecular differences based on DNA–DNA hybridization (relatedness of <46.5 % with the two species of the genus ), multilocus sequence analysis, and phenotypic and chemotaxonomic evidence separated strain JA248 from other species of the genus . Strain JA248 therefore represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JA248 ( = JCM 14795  = DSM 19786).

Funding
This study was supported by the:
  • , Department of Biotechnology, Govt. of India
  • , CSIR, Government of India
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.025965-0
2011-08-01
2020-08-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/8/1811.html?itemId=/content/journal/ijsem/10.1099/ijs.0.025965-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
    [Google Scholar]
  2. Biebl H., Pfennig N. 1981; Isolation of members of the family Rhodospirillaceae . In The Prokaryotes pp. 267–273 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. New York: Springer; [CrossRef]
    [Google Scholar]
  3. Castresana J. 2000; Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17:540–552[PubMed] [CrossRef]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  5. Don R. H., Cox P. T., Wainwright B. J., Baker K., Mattick J. S. 1991; ‘Touchdown’ PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19:4008 [CrossRef][PubMed]
    [Google Scholar]
  6. Drews G., Giesbrecht P. 1966; Rhodopseudomonas viridis, nov. spec., ein neu isoliertes, obligat phototrophes Bakterium. Arch Mikrobiol 53:255–262 [CrossRef]
    [Google Scholar]
  7. Hiraishi A. 1997; Transfer of the bacteriochlorophyll b-containing phototrophic bacteria Rhodopseudomonas viridis and Rhodopseudomonas sulfoviridis to the genus Blastochloris gen. nov. Int J Syst Bacteriol 47:217–219 [CrossRef][PubMed]
    [Google Scholar]
  8. Hiraishi A., Hoshino Y. 1984; Distribution of rhodoquinones in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 30:435–448 [CrossRef]
    [Google Scholar]
  9. Imhoff J. F. 1984; Quinones of phototrophic purple bacteria. FEMS Microbiol Lett 25:85–89 [CrossRef]
    [Google Scholar]
  10. Keppen O. I., Gorlenko V. M. 1975; [Characteristics of a new species of budding purple bacteria containing bacteriochlorophyll b]. Mikrobiologiya 44:258–264 (in Russian) [PubMed]
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74:5463–5467 [CrossRef][PubMed]
    [Google Scholar]
  14. Santos S. R., Ochman H. 2004; Identification and phylogenetic sorting of bacterial lineages with universally conserved genes and proteins. Environ Microbiol 6:754–759 [CrossRef][PubMed]
    [Google Scholar]
  15. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  16. Serrano W., Amann R., Rosselló-Mora R., Fischer U. 2010; Evaluation of the use of multilocus sequence analysis (MLSA) to resolve taxonomic conflicts within the genus Marichromatium . Syst Appl Microbiol 33:116–121 [CrossRef][PubMed]
    [Google Scholar]
  17. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M. 1994; A place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  19. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  21. Tourova T. P., Antonov A. S. 1988; Identification of microorganisms by rapid DNA-DNA hybridization. Methods in Microbiol 19:333–355 [CrossRef]
    [Google Scholar]
  22. Trüper H. G., Pfennig N. 1981; Isolation of members of the families Chromatiaceae and Chlorobiaceae . In The Prokaryotes: a Handbook on Habitats, Isolation and Identification of Bacteria pp. 279–289 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. New York: Springer;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.025965-0
Loading
/content/journal/ijsem/10.1099/ijs.0.025965-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error