1887

Abstract

A novel Gram-negative, motile, bacteriochlorophyll -containing purple non-sulfur bacterium, strain JA248, was isolated from phototrophic enrichments of a yellow–green epilithic biofilm sample collected from Gulmarg, India. The genomic DNA GC content of strain JA248 was 63.8 mol%. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain JA248 had highest similarity to members of the genus and was closely related to DSM 729 (98.5 % sequence similarity) and DSM 133 (98.4 %) of the class . Strain JA248 was characterized based on polyphasic taxonomy, and distinct phenotypic and molecular differences based on DNA–DNA hybridization (relatedness of <46.5 % with the two species of the genus ), multilocus sequence analysis, and phenotypic and chemotaxonomic evidence separated strain JA248 from other species of the genus . Strain JA248 therefore represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JA248 ( = JCM 14795  = DSM 19786).

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2011-08-01
2019-10-22
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. Yellow–green biofilm of an epilithic sample, from where strain JA248 was isolated.

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Phase-contrast photomicrographs of strain JA248 (a), DSM 133 (b) and DSM 729 (c). Bars, 5 µm.

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Electron photomicrograph of a negatively stained cell of strain JA248 showing polar flagella. Bar, 500 nm

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Transmission electron photomicrographs of horizontal (a) and longitudinal (b) sections of a cell of strain JA248 showing lamellar intracytoplasmic membrane structures. Bars, 150 nm (a), 250 nm (b).

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Whole-cell absorption spectra of strains JA248 (1), DSM 133 (2) and DSM 729 (3).

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