1887

Abstract

Four bacterial strains, SL014B-41A4, SL014B-20A1, SL014B-76A1 and SL014B-79A, isolated from a crude oil-contaminated saline soil of Shengli Oilfield, China, were investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SL014B-41A4 belonged to the genus in the order , with the highest sequence similarity with YIM YD3 (98.3 %). The DNA–DNA relatedness of strain SL014B-41A4 to YIM YD3 was 27.03±3.0 %. Strain SL014B-41A4 was Gram-negative staining, facultatively anaerobic and produced deep red pigment in artificial seawater medium. Cells of strain SL014B-41A4 were rod-shaped (0.6–4.0×1.25–25 µm), motile with a single polar flagellum and often formed branches. The strain contained Q-10 as the predominant respiratory ubiquinone and Cω7 (57.5 %), C (16.4 %) and 10-methyl C (9.1 %) as the major fatty acids. Strains SL014B-20A1, SL014B-76A1 and SL014B-79A were actinobacteria and belonged to the genus in the family of the order with the highest 16S rRNA gene sequence similarities with SST-39 (96.4 %), KISS-17Se (96.2 %) and Ben 106 (94.7 %). Strains SL014B-20A1, SL014B-76A1 and SL014B-79A were Gram-positive staining, facultatively anaerobic, non-endospore-forming, non-motile, acid-fast and oval to rod-shaped (0.48×0.5–1.0 µm). These three novel strains had -diaminopimelic acid (DAP) as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-9(H) as the only menaquinone and anteiso-C (67.11–76.14 %) as the major cellular fatty acid. The G+C contents of the genomic DNA of strain SL014B-41A4 and strains SL014B-20A1, SL014B-76A1 and SL014B-79A were 67.68 mol% and 65.65–67.17 mol%, respectively. Based on phenotypic and genotypic characteristics, strain SL014B-41A4 represents a novel species of the genus , for which the name is proposed, with strain SL014B-41A4 ( = DSM 22962 = CGMCC 1.9161) as the type strain. Strains SL014B-20A1, SL014B-76A1 and SL014B-79A represent a novel species of the genus , for which the name is proposed, with strain SL014B-20A1 ( = DSM 22955 = CGMCC 1.9159) as the type strain.

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2011-08-01
2020-01-23
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vol. , part 8, pp. 1767 - 1775

Neighbour-joining tree based on 16S rRNA gene sequences showing the relationship of strain SL014B-41A4T with most recognized species of the order and related taxa. Bootstrap values >70% are shown at nodes. Bar, 1 nt substitution per 100 nt. Maximum-likelihood tree based on 16S rRNA gene sequences showing the relationship of strain SL014B-41A4 with representatives of the order and related taxa. Maximum-parsimony tree based on 16S rRNA gene sequences showing the relationship of strain SL014B-41A4 with representatives of the order and related taxa. Bootstrap values >70% are shown at nodes. TLC plate image of polar lipids of strain SL014B-41A4 . Morphological features of cells of strain SL014B-41A4 observed by scanning electron microscopy. Bar, 5.0 µm. Neighbour-joining tree based on 16S rRNA gene sequences showing the relationship of strains SL014B-20A1 , SL014B-76A1, SL014B-79A with most recognized species of the family . Bootstrap values >70% are shown at nodes. Bar, 1 nt substitution per 100 nt. Maximum-likelihood tree based on 16S rRNA gene sequences showing the relationship of strains SL014B-20A1 , SL014B-76A1, SL014B-79A with representatives of the family and related taxa. Maximum-parsimony tree based on 16S rRNA gene sequences showing the relationship of strains SL014B-20A1 , SL014B-76A1, SL014B-79A with representatives of the family and related taxa. Bootstrap values >70% are shown at nodes. TLC plate image of polar lipids of strain SL014B-20A1 . Morphological features of cells of strain SL014B-20A1 and negatively stained cells observed by scanning electron microscopy. Bars, 5.0 µm (a); 500 nm (b).

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